Biomolecule-immobilized plate and method for fabricating biomolecule-immobilized plate

Abstract

There is provided a biomolecule-immobilized plate having a flat part, at least a surface of the flat part comprising a polymer material, wherein the flat part has an arithmetic average roughness of 0.1 nm˜5 nm, and a portion of the flat part has functional groups. Further, the biomolecule-immobilized plate is fabricated by irradiating the flat part of the plate surface which comprises the polymer material, with an ultraviolet ray under ozone atmosphere to produce functional groups having bonding ability to the biomolecules on a portion of the flat part. Thereby, it is possible to provide a biomolecule-immobilized plate having a surface of a high degree of smoothness on which functional groups are produced, a biomolecule-immobilized plate on which biomolecules are immobilized via functional groups, and methods for fabricating these biomolecule-immobilized plates easily and inexpensively.

1 . A biomolecule-immobilized plate having a flat part, at least a surface of the flat part comprising a polymer material, wherein said flat part has an arithmetic average roughness of 0.1 nm˜5 nm, and a portion of said flat part has functional groups. 
     
     
         2 . A biomolecule-immobilized plate having a flat part, at least a surface of the flat part comprising a polymer material, wherein said flat part has an arithmetic average roughness of 0.1 nm˜5 nm, and a portion of said flat part has carboxyl groups. 
     
     
         3 . A biomolecule-immobilized plate having a flat part, at least a surface of the flat part comprising a polymer material, wherein said flat part has an arithmetic average roughness of 0.1 nm˜5 nm, and a portion of said flat part has biomolecules which are bonded thereto via functional groups and protective groups which protect those functional groups that have no biomolecules bonded thereto. 
     
     
         4 . A biomolecule-immobilized plate having a flat part, at least a surface of the flat part comprising a polymer material, wherein said flat part has an arithmetic average roughness of 0.1 nm˜5 nm, and a portion of an arbitrary-shaped area in the flat part has biomolecules which are bonded thereto via functional groups and protective groups which protect those functional groups that have no biomolecules bonded thereto. 
     
     
         5 . A biomolecule-immobilized plate having a flat part, at least a surface of the flat part comprising a polymer material, wherein said flat part has an arithmetic average roughness of 0.1 nm˜5 nm, and a portion of said flat part has biomolecules which are bonded thereto via carboxyl groups and protective groups which protect those carboxyl groups that have no biomolecules bonded thereto. 
     
     
         6 . A biomolecule-immobilized plate having a flat part, at least a surface of the flat part comprising a polymer material, wherein said flat part has an arithmetic average roughness of 0.1 nm˜5 nm, and a portion of an arbitrary-shaped area in the flat part has biomolecules which are bonded thereto via carboxyl groups and protective groups which protect those carboxyl groups that have no biomolecules bonded thereto. 
     
     
         7 . A biomolecule-immobilized plate as defined in any of  claims 1  to  6 , wherein said polymer material is one selected from a group consisting of polymethyl methacrylate, polyethylene terephthalate, and polycarbonate. 
     
     
         8 . A biomolecule-immobilized plate as defined in any of  claims 3  to  6 , wherein said protective groups are amino groups. 
     
     
         9 . A biomolecule-immobilized plate as defined in any of  claims 1  to  6 , wherein said biomolecules are one selected from a group consisting of nucleic acid, protein, and peptide. 
     
     
         10 . A method for fabricating a biomolecule-immobilized plate comprising preparing a plate having a flat part, at least a surface of the flat part comprising a polymer material; and irradiating the flat part with an ultraviolet ray under ozone atmosphere to produce, on a portion of the flat part, functional groups having bonding ability to the biomolecules. 
     
     
         11 . A method for fabricating a biomolecule-immobilized plate comprising preparing a plate having a flat part, at least a surface of the flat part comprising a polymer material; irradiating the flat part with an ultraviolet ray under ozone atmosphere to produce carboxyl groups on a portion of the flat part; immobilizing biomolecules onto the flat part via the carboxyl groups; and adding protective groups for protecting carboxyl groups which have no biomolecules bonded thereto. 
     
     
         12 . A method for fabricating a biomolecule-immobilized plate as defined in  claims 10  or  11  wherein a mask which exposes an arbitrary area of the flat part is disposed, and the arbitrary area is irradiated with an ultraviolet ray under ozone atmosphere. 
     
     
         13 . A method for fabricating a biomolecule-immobilized plate as defined in  claim 10  or  11  wherein the irradiation energy of the ultraviolet ray is larger than the bonding energy of carbon-carbon bonds in the polymer material which constitutes the flat part. 
     
     
         14 . A method for fabricating a biomolecule-immobilized plate as defined in  claim 10  or  11  wherein said polymer material is one selected from a group consisting of polymethyl methacrylate, polyethylene terephthalate, and polycarbonate. 
     
     
         15 . A method for fabricating a biomolecule-immobilized plate as defined in  claim 11  wherein said protective groups are amino groups. 
     
     
         16 . A method for fabricating a biomolecule-immobilized plate as defined in  claim 10  or  11  wherein said biomolecules are one selected from a group consisting of nucleic acid, protein, and peptide.