Diagnosis and treatment of PTP related disorders

Abstract

The present invention relates to PTP05 polypeptides and PTP10 polypeptides, nucleic acids encoding such polypeptides, cells, tissues and animals containing such nucleic acids, antibodies to such polypeptides, assays utilizing such polypeptides, and methods relating to all of the foregoing. Methods for treatment, diagnosis, and screening are provided for PTP05 and/or PTP10 related diseases or conditions characterized by an abnormal interaction between a PTP05 polypeptide and a PTP05 binding partner and/or a PTP10 polypeptide and a PTP10 binding partner.

1 . An antibody or antibody fragment having specific binding affinity to (i) a PTP05 polypeptide, (ii) a PTP10 polypeptide, (iii) a PTP05 domain polypeptide, or (iv) a PTP10 domain polypeptide.  
     
     
         2 . The antibody or antibody fragment of  claim 1 , wherein the PTP05 polypeptide comprises a sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO:6 and SEQ ID NO:7.  
     
     
         3 . The antibody or antibody fragment of  claim 1 , wherein the PTP10 polypeptide comprises the sequence of SEQ ID NO:8.  
     
     
         4 . The antibody or antibody fragment of  claim 1 , wherein the PTP05 domain or the PTP10 domain is a portion of any one of SEQ ID NOs: 5, 6, 7, or 8 that is homologous in sequence to an amino acid sequence from a known protein that is predictive of a common function, interaction or activity.  
     
     
         5 . The antibody or antibody fragment of  claim 4 , wherein the PTP05 domain or the PTP10 domain is homologous in sequence to the amino acid sequence of (i) an Src Homology 2 domain, (ii) an Src Homology 3 domain, or (iii) a pleckstrin domain.  
     
     
         6 . The antibody or antibody fragment of  claim 4 , wherein the PTP05 domain or the PTP10 domain has a relative homology of at least 90% to the amino acid sequence from the known protein.  
     
     
         7 . The antibody or antibody fragment of  claim 6 , wherein the PTP05 domain or the PTP10 domain has a relative homology of at least 95% to the amino acid sequence from the known protein.  
     
     
         8 . The antibody or antibody fragment of  claim 1 , wherein the sequence of the PTP05 polypeptide comprises (A) the full length amino acid sequence of the sequence set forth in (i) SEQ ID NO:5 lacking one or more of the following segments of amino acid residues: 1-187, 188-420, or 421-426; (ii) SEQ ID NO:6 lacking one or more of 44-80, 225-457, or 458-463, or (iii) SEQ ID NO:7 lacking one or more of 1-87, 188-405, or 406-412; 
 (B) (i) residues 1-187, 188-420, or 421-426 of SEQ ID NO:5, (ii) residues 44-80, 225-457, 458-463 of SEQ ID NO:6, or (iii) residues 1-87, 188-405, 406-412 of SEQ ID NO:7; or    (C) the full length amino acid sequence set forth in SEQ ID NO:5, SEQ ID NO:6, or SEQ ID NO:7 except that the sequence lacks one or more of the domains selected from the group consisting of (i) an N-terminal domain, (ii) a catalytic domain, and (iii) a C-terminal domain.    
     
     
         9 . The antibody or antibody fragment of  claim 1 , wherein the sequence of the PTP10 polypeptide comprises the full length amino acid sequence set forth in SEQ ID NO:8 except that the sequence of the PTP10 polypeptide lacks one or more of the domains selected from the group consisting of (i) an N-terminal domain, (ii) a catalytic domain, and (iii) a C-terminal domain.  
     
     
         10 . The antibody or antibody fragment of  claim 1 , wherein the antibody is a polyclonal antibody or a monoclonal antibody.  
     
     
         11 . A kit, comprising (i) a first container containing the antibody of  claim 1 , and (ii) a second container containing a conjugate that comprises (a) a binding partner of the antibody and (b) a label.  
     
     
         12 . The kit of  claim 11 , further comprising one or more additional containers comprising (i) a wash reagent and (ii) one or more reagents for detecting the presence of the bound antibody in a sample.