US2008004437A1PendingUtilityA1

Cloned Genes Encoding Reverse Transcriptase lacking RNase H Activity

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Assignee: INVITROGEN CORPPriority: Jan 13, 1988Filed: May 29, 2007Published: Jan 3, 2008
Est. expiryJan 13, 2008(expired)· nominal 20-yr term from priority
Inventors:Michael KotewiczGary F. Gerard
C12P 19/34C12N 9/1276C12Q 1/68C12Y 207/07049Y10S435/81Y10S435/975
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Claims

Abstract

The invention relates to a gene which encodes reverse transcriptase having DNA polymerase activity and substantially no RNase H activity. The invention also relates to vectors containing the gene and hosts transformed with the vectors of the invention. The invention also relates to a method of producing reverse transcriptase having DNA polymerase activity and substantially no RNase H activity by expressing the reverse transcriptase genes of the present invention in a host. The invention also relates to a method of producing cDNA from mRNA using the reverse transcriptase of the invention. The invention also relates to a kit for the preparation of cDNA from mRNA comprising the reverse transcriptase of the invention.

Claims

exact text as granted — not AI-modified
1 . A gene which encodes reverse transcriptase having DNA polymerase activity and substantially no RNase H activity.  
   
   
       2 - 23 . (canceled)

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