Reagent for determining laminin 5 antigen in biological sample and assay method

Abstract

A method of determining a laminin 5 antigen in a biological sample, comprising the steps of bringing an antibody reactive to a laminin 5 γ2 chain N-terminal fragment into contact with the biological sample; measuring a reaction of the antibody; and determining an amount of the laminin 5 antigen based on a measurement result of the reaction, as well as, a method of detecting a laminin 5-producing tumor cell, a method of examining acute respiratory distress syndrome and a method of evaluating malignancy of a malignant tumor based on the assay method.

1 . A method of determining β4 integrin expression, comprising steps of: 
 bringing an antibody reactive to a laminin 5 into contact with a cell culture supernatant; measuring a reaction of the antibody; and    determining an expression amount of a β4 integrin in the cell based on the measurement result of the reaction.    
   
   
       2 . The method of  claim 1 , wherein the antibody is a monoclonal antibody produced from a cell deposited with an accession number selected from the group consisting of: FERM BP-8133, FERM BP-8134, FERM BP-8135, FERM BP-8136, FERM BP-8137, FERM BP-8140, and combinations thereof.