32 Human Secreted Proteins

Abstract

The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating disorders related to these novel human secreted proteins.

1 . An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of: 
 (a) a polynucleotide fragment of SEQ ID NO:X or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No: Z, which is hybridizable to SEQ ID NO:X;    (b) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:Y or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No: Z, which is hybridizable to SEQ ID NO:X;    (c) a polynucleotide encoding a polypeptide domain of SEQ ID NO:Y or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No: Z, which is hybridizable to SEQ ID NO:X;    (d) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:Y or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No: Z, which is hybridizable to SEQ ID NO:X;    (e) a polynucleotide encoding a polypeptide of SEQ ID NO:Y or the cDNA sequence included in ATCC Deposit No: Z, which is hybridizable to SEQ ID NO:X, having biological activity;    (f) a polynucleotide which is a variant of SEQ ID NO:X;    (g) a polynucleotide which is an allelic variant of SEQ ID NO:X;    (h) a polynucleotide which encodes a species homologue of the SEQ ID NO:Y;    (i) a polynucleotide capable of hybridizing under stringent conditions to any one of the polynucleotides specified in (a)-(h), wherein said polynucleotide does not hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence of only A residues or of only T residues.    
     
     
         2 . The isolated nucleic acid molecule of  claim 1 , wherein the polynucleotide fragment comprises a nucleotide sequence encoding a secreted protein.  
     
     
         3 . The isolated nucleic acid molecule of  claim 1 , wherein the polynucleotide fragment comprises a nucleotide sequence encoding the sequence identified as SEQ ID NO:Y or the polypeptide encoded by the cDNA sequence included in ATCC Deposit No: Z, which is hybridizable to SEQ ID NO:X.  
     
     
         4 . The isolated nucleic acid molecule of  claim 1 , wherein the polynucleotide fragment comprises the entire nucleotide sequence of SEQ ID NO:X or the cDNA sequence included in ATCC Deposit No: Z, which is hybridizable to SEQ ID NO:X.  
     
     
         5 . A recombinant vector comprising the isolated nucleic acid molecule of  claim 1 .  
     
     
         6 . A method of making a recombinant host cell comprising the isolated nucleic acid molecule of  claim 1 .  
     
     
         7 . A recombinant host cell produced by the method of  claim 6 .  
     
     
         8 . The recombinant host cell of  claim 7  comprising vector sequences.  
     
     
         9 . An isolated polypeptide comprising an amino acid sequence at least 95% identical to a sequence selected from the group consisting of: 
 (a) a polypeptide fragment of SEQ ID NO:Y or the encoded sequence included in ATCC Deposit No: Z;    (b) a polypeptide fragment of SEQ ID NO:Y or the encoded sequence included in ATCC Deposit No: Z, having biological activity;    (c) a polypeptide domain of SEQ ID NO:Y or the encoded sequence included in ATCC Deposit No: Z;    (d) a polypeptide epitope of SEQ ID NO:Y or the encoded sequence included in ATCC Deposit No: Z;    (e) a secreted form of SEQ ID NO:Y or the encoded sequence included in ATCC Deposit No: Z;    (f) a full length protein of SEQ ID NO:Y or the encoded sequence included in ATCC Deposit No: Z;    (g) a variant of SEQ ID NO:Y;    (h) an allelic variant of SEQ ID NO:Y; or    (i) a species homologue of the SEQ ID NO:Y.    
     
     
         10 . The isolated polypeptide of  claim 9 , wherein the secreted form or the full length protein comprises sequential amino acid deletions from either the C-terminus or the N-terminus.  
     
     
         11 . An isolated antibody that binds specifically to the isolated polypeptide of  claim 9 .  
     
     
         12 . A recombinant host cell that expresses the isolated polypeptide of  claim 9 .  
     
     
         13 . A method of making an isolated polypeptide comprising: 
 (a) culturing the recombinant host cell of  claim 7  under conditions such that said polypeptide is expressed; and    (b) recovering said polypeptide.    
     
     
         14 . The polypeptide produced by  claim 13 .  
     
     
         15 . A method for preventing, treating, or ameliorating a medical condition, comprising administering to a mammalian subject a therapeutically effective amount of the polynucleotide of  claim 1 .  
     
     
         16 . A method for preventing, treating, or ameliorating a medical condition, comprising administering to a mammalian subject a therapeutically effective amount of the polypeptide of  claim 9 .  
     
     
         17 . A method for preventing, treating, or ameliorating a medical condition, comprising administering to a mammalian subject a therapeutically effective amount of the antibody of  claim 11 .  
     
     
         18 . A method of diagnosing a pathological condition or a susceptibility to a pathological condition in a subject comprising: 
 (a) determining the presence or absence of a mutation in the polynucleotide of  claim 1;  and    (b) diagnosing a pathological condition or a susceptibility to a pathological condition based on the presence or absence of said mutation.    
     
     
         19 . A method of diagnosing a pathological condition or a susceptibility to a pathological condition in a subject comprising: 
 (a) determining the presence or amount of expression of the polypeptide of  claim 9  in a biological sample; and    (b) diagnosing a pathological condition or a susceptibility to a pathological condition based on the presence or amount of expression of the polypeptide.    
     
     
         20 . A method for identifying a binding partner to the polypeptide of  claim 9  comprising: 
 (a) contacting the polypeptide of  claim 9  with a binding partner; and    (b) determining whether the binding partner effects an activity of the polypeptide.