Method for producing a cell culture substrate

Abstract

A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells. To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.

1 - 16 . (canceled)  
     
     
         17 . The method for producing a cell culture substrate according to claim  1 , wherein the region-selecting applying method is an ink-jetting method.  
     
     
         18 . An ink-jetting cell-containing liquid comprising cells, a culture solution and a biological cell adhesive material, and jets out in a pattern form onto a substrate by an ink-jetting method, thereby forming a cell culture substrate onto which the cells adhere in the pattern form.  
     
     
         19 . An ink-jetting cell-containing liquid jets out in a pattern form onto a substrate by an ink-jetting method to form a cell culture substrate onto which cells adhere in the pattern form, and the cells being micro-encapsulated.  
     
     
         20 . The ink-jetting cell-containing liquid according to  claim 18 , which is the cell-containing liquid used in the method for producing a cell culture substrate according to  claim 17 .  
     
     
         21 . The ink-jetting cell-containing liquid according to  claim 19 , which is the cell-containing liquid used in the method for producing a cell culture substrate according to  claim 17 .  
     
     
         22 . An ink-jetting device for producing a cell culture substrate, comprising a cell-containing liquid supplying section into which a cell-containing liquid is filled, a jetting-out section having a piezoelectrically driving head unit for jetting out the cell-containing liquid supplied from the cell-containing liquid supplying section onto a cell culture patterning substrate, and a stage for fixing the cell culture patterning substrate, 
 wherein the cell-containing liquid supplying section is provided with a stirring means for dispersing cells homogeneously in the cell-containing liquid filled, and    the jetting-out section and the stage are provided with one or more temperature controlling means for keeping a temperature of the cell-containing liquid constant before and after a jetting-out.    
     
     
         23 . The ink-jetting device for producing a cell culture substrate according to  claim 22 , which is provided with a humidity controlling means for controlling a humidity between the piezoelectrically driving head unit and the cell culture patterning substrate fixed onto the stage.