Nuclear matrix protein alterations associated with colon cancer and colon metastasis to the liver and uses thereof

Abstract

Proteins useful in the diagnosis of proliferative disorders of the colon are present in nuclear matrix protein preparations and can be characterized by molecular weight, isoelectric point, and amino acid sequence. The proteins may be identified, for example, by 2D-gel electrophoresis or by specific binding partners, such as antibodies.

1 . A method of analyzing a biological specimen for the presence of an analyte indicative of a clinical condition, comprising the steps of: 
 contacting the specimen with an antibody which binds to the analyte,    detecting binding of the antibody in the specimen, and    correlating the detected binding to the clinical condition,    wherein the biological specimen is a body fluid, the antibody binds to Ile-Leu-Leu-Phe-Asp-Tyr-Phe-Asn-Arg (SEQ ID NO: 28), and the detected binding is correlated to a cell-proliferative disorder of the colon.