US2007238115A1PendingUtilityA1

Method of Diagnosing and Treating Colon Cancer

38
Individually held — no corporate assignee on recordPriority: Feb 27, 2006Filed: Feb 27, 2007Published: Oct 11, 2007
Est. expiryFeb 27, 2026(expired)· nominal 20-yr term from priority
Inventors:Michael B. DwinellPriscilla A. JohanesenMichael K. Wendt
C12Q 2523/125C12Q 1/6886C12Q 2600/154A61K 38/17A61P 35/00C12Q 2600/118
38
PatentIndex Score
0
Cited by
0
References
0
Claims

Abstract

The invention provides a method of diagnosing carcinoma in a patient, the method comprising providing a sample of colorectal cells from a human patient and analyzing the sample for CXCL12 hypermethylation.

Claims

exact text as granted — not AI-modified
1 . A method of diagnosing carcinoma in a patient, the method comprising providing a sample of colorectal cells from a human patient and analyzing the sample for CXCL12 gene hypermethylation or hypermethylation up-stream from the CXCL12 gene, wherein hypermethylation of the CXCL12 gene or up-stream region indicates a diagnosis of colon cancer.  
     
     
         2 . The method of  claim 1  wherein hypermethylation is observed via PCR analysis of the CXCL12 gene.  
     
     
         3 . The method of  claim 2  wherein CXCL12-M and CXCL12-U primer pairs (SEQ. ID NOs 1 and 2) are used in PCR analysis.  
     
     
         4 . The method of  claim 2  wherein primer pairs selected from SEQ. ID NOs 1-16 are used in PCR analysis.  
     
     
         5 . The method of  claim 1  wherein the CXCL12 gene region between −275 and −35 is examined for hypermethylation.  
     
     
         6 . The method of  claim 1  wherein the CXCL12 gene regions between −60 and −38 or −12 and +1 are examined for hypermethylation.  
     
     
         7 . The method of  claim 1  wherein the CXCL12 up stream region is −1877 to −1581, −1391 to −1231 or −1123 to −899.  
     
     
         8 . The method of  claim 1  wherein the cells are obtained from stool specimens.  
     
     
         9 . The method of  claim 1  wherein the cells are obtained from surgical resection or biopsy tissue.  
     
     
         10 . A method of treating carcinoma in a patient, the method comprising administering to the patient a therapeutically effective amount of CXCL12 protein.  
     
     
         11 . The method of  claim 10  wherein the method of administering CXCL12 protein is through recombinant DNA technique.  
     
     
         12 . The method of  claim 10  wherein the method of administration of CXCL12 is via native protein.  
     
     
         13 . A method of treating carcinoma in a cell, the method comprising administering to the patient a therapeutically effective amount of CXCL12 protein.  
     
     
         14 . The method of  claim 13  wherein the method of administering CXCL12 protein is through recombinant DNA technique.  
     
     
         15 . The method of  claim 13  wherein the method of administration of CXCL12 is via native protein.  
     
     
         16 . A diagnostic array for diagnosing tumors, the array comprising a multi-gene array comprising the CXCL12 gene and at least one gene selected from the group of P15 (CDKNZB), hMLH1 and APC.  
     
     
         17 . The diagnostic array of  claim 16  comprising CXCL12, P15 (CDKNZB), hMLH1 and APC genes.  
     
     
         18 . A diagnostic array for diagnosing tumors, the array comprising primers diagnostic for CXCL12 gene or up-stream region hypermethylation.

Join the waitlist — get patent alerts

Track US2007238115A1 — get alerts on status changes and closely related new filings.

We store only your email — no account needed. See our privacy policy.