Osteogenic Composite Matrix, Method for the Production Thereof and Implant and Scaffold for Tissue Engineering Provided with a Coating Formed by Said Osteogenic Composite matrix

Abstract

The invention relates to an osteogenic composite matrix consisting of collagen and non-collagen components of an extracellular matrix (ECM-components), to a method for producing said matrix, to a method for producing an implant or a scaffold for tissue engineering which is provided with a coating formed by said osteogenic composite matrix and is used for stimulating and accelerating a hard tissue formation such as, for example the implant osseointegration in bones. The inventive osteogenic composite matrix comprises a collagen and at least one non-collagen ECM component or the derivatives thereof, wherein the collagen component consists of non-crosslinked collagen fibres produced by fibrillogenesis and the non-collagen ECM component or the derivatives thereof are integrated into said collagen fibres.

1 - 13 . (canceled)  
   
   
       14 . Osteogenic matrix composite of collagen and at least one noncollagenic ECM component or its derivatives, wherein the collagen component consists of noncrosslinked collagen fibrils produced by means of fibrillogenesis and in that i-o this are integrated the at least one noncollagenic ECM component or its derivatives.  
   
   
       15 . The osteogenic matrix composite as claimed in  claim 14 , wherein the noncollagenic ECM components contain glycosaminoglycans.  
   
   
       16 . The osteogenic matrix composite as claimed in  claim 15 , wherein the noncollagenic ECM component contains chondroitin sulfate of type A, C, D, or E, dermatan sulfate, keratan sulfate, heparan sulfate, heparin, hyaluronic acid and their derivatives, individually or mixed.  
   
   
       17 . The osteogenic matrix composite as claimed in  claim 14 , wherein the noncollagenic ECM component contains noncollagenic matrix proteins.  
   
   
       18 . The osteogenic matrix composite as claimed in  claim 17 , wherein the noncollagenic ECM component contains, as noncollagenic matrix proteins, fibronectin, decorin, biglycan, laminin, versican individually or mixed.  
   
   
       19 . The osteogenic matrix composite as claimed in  claim 14 , wherein the collagen component consists of one of the collagens I, II, III, V, IX, XI, or combinations thereof.  
   
   
       20 . A method for the production of an osteogenic matrix composite of collagen and at least one noncollagenic ECM component or its derivatives, wherein collagen fibrils are produced by means of fibrillogenesis, in that before fibrillogenesis the at least one noncollagenic ECM component or its derivatives is added, and in that the collagen fibrils produced in this way are resuspended in water or in a buffer system and optionally lyophilized.  
   
   
       21 . The method as claimed in  claim 20 , wherein the flbrillogenesis is carried out under the conditions, temperature range from 4° C. to 40° C., preferably 25° C. to 37° C., collagen concentration of 5C to 5000 pg/ml, preferably 250 to 1000 pg/ml, pH 4 to pH 9, preferably pH 6 to pH 8, phosphate content up to 500 mmol/l, preferably 30 to 60 mmol/l, NaCl content up to 1000 mmol/l, preferably up to 300 mmol/l.  
   
   
       22 . The use of an osteogenic matrix composite as claimed in  claim 14  for the coating of implants or scaffolds for tissue engineering.  
   
   
       23 . A method for the production of an implant or of a scaffold for tissue engineering having a coating of an osteogenic matrix composite as claimed in  claim 14 , wherein collagen fibrils are produced by means of fibrillogenesis, in that before fibrillogenesis the at least one noncollagenic ECM component or its derivatives are added, and in that the collagen fibrils produced in this way are resuspended in water or in a buffer and subsequently immobilized on the surface of the implant or of the scaffold in a dip-coating process.  
   
   
       24 . A method for the production of a scaffold for tissue engineering having a coating of an osteogenic matrix composite as claimed in  claim 14 , wherein collagen fibrils are produced by means of fibrillogenesis, in that before fibrillogenesis the at least one noncollagenic ECM component or its derivatives are added in such a way that fibril formation is induced in the scaffold, where the fibrils formed in situ either remain as a gel or are dried.  
   
   
       25 . An implant or scaffold for tissue engineering having a coating of an osteogenic matrix composite as claimed in  claim 14 .  
   
   
       26 . A coating solution comprising an osteogenic matrix composite as claimed in  claim 14.