Ribonucleases and methods of making them recombinantly

Abstract

Methods for recombinantly producing new RNases, as well as previously-known RNases, are disclosed. The new RNases are actable against human carcinoma cells.

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         10 . A protein having the amino acid sequence of SEQ ID NO:60 produced recombinantly.  
     
     
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         20 . A protein having the amino acid sequence of SEQ ID NO:60.  
     
     
         21 . A conservatively modified variant of the protein of  claim 20 .  
     
     
         22 . A gene that when expressed in a host encodes the protein of  claim 20 .  
     
     
         23 . A gene that when expressed in a host encodes the protein of  claim 21 .  
     
     
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         42 . A protein having the amino acid sequence of SEQ ID NO:17 preceded at its N-terminal end by a leader sequence that has at least the first and at most all of the residues of SEQ ID NO:64 in order.  
     
     
         43 . A protein having the amino acid sequence of SEQ ID NO:17 preceded at its N-terminal end by a leader sequence that has at least the first and at most all of the residues of SEQ ID NO:66 in order.  
     
     
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         57 . A vector containing DNA of SEQ ID NO:18 encoding the Ribonuclease of SEQ ID NO:17.