Ribonucleases and methods of making them recombinantly

Abstract

Methods for recombinantly producing new RNases, as well as previously-known RNases, are disclosed. The new RNases are active against human carcinoma cells.

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         14 . A protein having the amino acid sequence of SEQ ID NO:68 produced recombinantly.  
     
     
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         33 . A protein having the amino acid sequence of SEQ ID NO:68.  
     
     
         34 . A conservatively modified variant of the protein of  claim 33 .  
     
     
         35 . A gene that when expressed in a host encodes the protein of  claim 33 .  
     
     
         36 . A gene that when expressed in a host encodes the protein of  claim 34 .  
     
     
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         46 . A protein having the amino acid sequence of SEQ ID NO:51 preceded at its N-terminal end by a leader sequence that has at least the first and at most all of the residues of SEQ ID NO:64 in order.  
     
     
         47 . A protein having the amino acid sequence of SEQ ID NO:51 preceded at its N-terminal end by a leader sequence that has at least the first and at most all of the residues of SEQ ID NO:66 in order.  
     
     
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         57 . A vector containing DNA of SEQ ID NO:52 encoding the Ribonuclease of SEQ ID NO:51.