Complete nutrient medium for use as a cosmetic and cosmetic use thereof

Abstract

A cosmetic composition contains an aqueous complex nutritive base comprising a plurality of amino acids, at least one vitamin, a plurality of assimilable organic components, and at least one inorganic salt. The cosmetic composition does not contain a biological extract of animal or cellular origin, or a living nourishing substrate. A cosmetic method comprises contacting human skin with the cosmetic composition.

1 . A method of cosmetic treatment, comprising contacting only an area of human skin whose integrity has not been breached by injury with a treatment composition comprising an aqueous complex nutritive base comprising: 
 a plurality of amino acids,    at least one vitamin,    a plurality of assimilable organic components each selected from the group consisting of i-Inositol, Putrescine 2HCl, Sodium pyruvate, Thymidine, Adenine (HCl), DL-Lipoic acid and D-Glucose, and    at least one inorganic salt,    wherein said treatment composition does not comprise a biological extract of animal or cellular origin, or a living nourishing substrate, or a cellular growth stimulating compound or factor, or a hormone.    
   
   
       2 . The method of  claim 1 , wherein the pH and osmolarity of said complex nutritive base are per se close to physiological conditions.  
   
   
       3 . The method of  claim 1 , wherein said treatment composition consists essentially of components that are biomimetic to skin.  
   
   
       4 . The method of  claim 1 , wherein said amino acids include at least one amino acid selected from the group consisting of L-Alanine, L-Arginine HCl, L-Asparagine, L-Aspartic acid, L-Cysteine HCl.H 2 O, L-Glutamic acid, L-Glutamine, Glycine, L-Histidine HCl.H 2 O, L-Isoleucine, L-Leucine, L-Lysine HCl, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine 2 Na 2H 2 O, and L-Valine.  
   
   
       5 . The method of  claim 1 , wherein said at least one vitamin includes at least one vitamin selected from the group consisting of d-Biotin, Folic acid, Nicotinamide, Ca D-Pantothenate, Pyridoxine HCl, Riboflavin, Thiamine HCl, and Vitamin B 12 .  
   
   
       6 . The method of  claim 1 , wherein said complex nutritive base supports per se viable in vitro growth of human epidermal keratinocytes, with no proliferation of any transformed human epidermal keratinocyte.  
   
   
       7 . The method of  claim 1 , wherein the complex nutritive base does not have per se cytotoxic manifestations to skin.  
   
   
       8 . The method of  claim 1 , wherein said method is for maintaining the integrity and balance of the superficial cells of the skin.  
   
   
       9 . A method of cosmetic treatment, comprising contacting only an area of human skin whose integrity has not been breached by a wound with a treatment composition comprising an aqueous complex nutritive base comprising: 
 a plurality of amino acids,    at least one vitamin,    a plurality of assimilable organic components each selected from the group consisting of i-Inositol, Putrescine 2HCl, Sodium pyruvate, Thymidine, Adenine (HCl), DL-Lipoic acid and D-Glucose, and    at least one inorganic salt,    wherein said treatment composition does not comprise a biological extract of animal or cellular origin, or a living nourishing substrate, or a cellular growth stimulating compound or factor, or a hormone.    
   
   
       10 . The method of  claim 9 , wherein the pH and osmolarity of said complex nutritive base are per se close to physiological conditions.  
   
   
       11 . The method of  claim 9 , wherein said treatment composition consists essentially of components that are biomimetic to skin.  
   
   
       12 . The method of  claim 9 , wherein said amino acids include at least one amino acid selected from the group consisting of L-Alanine, L-Arginine HCI, L-Asparagine, L-Aspartic acid, L-Cysteine HCl.H 2 O, L-Glutamic acid, L-Glutamine, Glycine, L-Histidine HCl.H 2 O, L-Isoleucine, L-Leucine, L-Lysine HCl, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine 2 Na 2H 2 O, and L-Valine.  
   
   
       13 . The method of  claim 9 , wherein said at least one vitamin includes at least one vitamin selected from the group consisting of d-Biotin, Folic acid, Nicotinamide, Ca D-Pantothenate, Pyridoxine HCl, Riboflavin, Thiamine HCl, and Vitamin B 12 .  
   
   
       14 . The method of  claim 9 , wherein said complex nutritive base supports per se viable in vitro growth of human epidermal keratinocytes, with no proliferation of any transformed human epidermal keratinocyte.  
   
   
       15 . The method of  claim 9 , wherein the complex nutritive base does not have per se cytotoxic manifestations to skin.  
   
   
       16 . The method of  claim 9 , wherein said method is for maintaining the integrity and balance of the superficial cells of the skin.  
   
   
       17 . A method of cosmetic treatment, comprising contacting human skin with a treatment composition comprising an aqueous complex nutritive base comprising: 
 a plurality of amino acids,    at least one vitamin,    a plurality of assimilable organic components each selected from the group consisting of i-Inositol, Putrescine 2HCl, Sodium pyruvate, Thymidine, Adenine (HCl), DL-Lipoic acid and D-Glucose, and    at least one inorganic salt,    wherein said treatment composition does not contain a biological extract of animal or cellular origin, or a living nourishing substrate, and wherein said treatment composition consists essentially of components that are biomimetic to skin.    
   
   
       18 . The method of  claim 17 , wherein said complex nutritive base supports per se viable in vitro growth of human epidermal keratinocytes.  
   
   
       19 . The method of  claim 17 , wherein the pH and osmolarity of said complex nutritive base are per se close to physiological conditions.  
   
   
       20 . The method of  claim 17 , wherein said amino acids include at least one amino acid selected from the group consisting of L-Alanine, L-Arginine HCI, L-Asparagine, L-Aspartic acid, L-Cysteine HCl.H 2 O, L-Glutamic acid, L-Glutamine, Glycine, L-Histidine HCl.H 2 O, L-Isoleucine, L-Leucine, L-Lysine HCl, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine 2 Na 2H 2 O, and L-Valine.  
   
   
       21 . The method of  claim 17 , wherein said at least one vitamin includes at least one vitamin selected from the group consisting of d-Biotin, Folic acid, Nicotinamide, Ca D-Pantothenate, Pyridoxine HCl, Riboflavin, Thiamine HCl, and Vitamin B 12 .  
   
   
       22 . The method of  claim 17 , wherein the complex nutritive base does not have per se cytotoxic manifestations to skin.  
   
   
       23 . The method of  claim 17 , wherein said method is for maintaining the integrity and balance of the superficial cells of the skin.  
   
   
       24 . A cosmetic composition, comprising an aqueous complex nutritive base comprising: 
 a plurality of amino acids,    at least one vitamin,    a plurality of assimilable organic components each selected from the group consisting of i-Inositol, Putrescine 2HCl, Sodium pyruvate, Thymidine, Adenine (HCl), DL-Lipoic acid and D-Glucose, and    at least one inorganic salt,    wherein said cosmetic composition does not comprise a biological extract of animal or cellular origin, or a living nourishing substrate, or a cellular growth stimulating compound or factor, or a hormone.    
   
   
       25 . The cosmetic composition of  claim 24 , wherein the pH and osmolarity of said complex nutritive base are per se close to physiological conditions.  
   
   
       26 . The cosmetic composition of  claim 24 , wherein said cosmetic composition consists essentially of components that are biomimetic to skin.  
   
   
       27 . The cosmetic composition of  claim 24 , wherein said amino acids include at least one amino acid selected from the group consisting of L-Alanine, L-Arginine HCl, L-Asparagine, L-Aspartic acid, L-Cysteine HCl.H 2 O, L-Glutamic acid, L-Glutamine, Glycine, L-Histidine HCl.H 2 O, L-Isoleucine, L-Leucine, L-Lysine HCl, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine 2 Na 2H 2 O, and L-Valine.  
   
   
       28 . The cosmetic composition of  claim 24 , wherein said at least one vitamin includes at least one vitamin selected from the group consisting of d-Biotin, Folic acid, Nicotinamide, Ca D-Pantothenate, Pyridoxine HCl, Riboflavin, Thiamine HCl, and Vitamin B 12 .  
   
   
       29 . The cosmetic composition of  claim 24 , wherein said complex nutritive base supports per se viable in vitro growth of human epidermal keratinocytes, with no proliferation of any transformed human epidermal keratinocyte.  
   
   
       30 . The cosmetic composition of  claim 24 , wherein the complex nutritive base does not have per se cytotoxic manifestations to skin.  
   
   
       31 . A cosmetic composition, comprising an aqueous complex nutritive base comprising: 
 a plurality of amino acids,    at least one vitamin,    a plurality of assimilable organic components each selected from the group consisting of i-Inositol, Putrescine 2HCl, Sodium pyruvate, Thymidine, Adenine (HCl), DL-Lipoic acid and D-Glucose, and    at least one inorganic salt,    wherein said cosmetic composition does not contain a biological extract of animal or cellular origin, or a living nourishing substrate, and wherein said cosmetic composition consists essentially of components that are biomimetic to skin.    
   
   
       32 . The cosmetic composition of  claim 31 , wherein said complex nutritive base supports per se viable in vitro growth of human epidermal keratinocytes.  
   
   
       33 . The cosmetic composition of  claim 31 , wherein the pH and osmolarity of said complex nutritive base are per se close to physiological conditions.  
   
   
       34 . The cosmetic composition of  claim 31 , wherein said amino acids include at least one amino acid selected from the group consisting of L-Alanine, L-Arginine HCl, L-Asparagine, L-Aspartic acid, L-Cysteine HCl.H 2 O, L-Glutamic acid, L-Glutamine, Glycine, L-Histidine HCl.H 2 O, L-Isoleucine, L-Leucine, L-Lysine HCl, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine 2 Na 2H 2 O, and L-Valine.  
   
   
       35 . The cosmetic composition of  claim 31 , wherein said at least one vitamin includes at least one vitamin selected from the group consisting of d-Biotin, Folic acid, Nicotinamide, Ca D-Pantothenate, Pyridoxine HCl, Riboflavin, Thiamine HCl, and Vitamin B 12 .  
   
   
       36 . The cosmetic composition of  claim 31 , wherein the complex nutritive base does not have per se cytotoxic manifestations to skin.  
   
   
       37 . The cosmetic composition of  claim 31 , said aqueous complex nutritive medium comprising at least four of said assimilable organic components.