US2007218463A1PendingUtilityA1

Rearranged squamous cell carcinoma antigen genes II

Assignee: ROJER EVAPriority: Mar 15, 2001Filed: Sep 12, 2003Published: Sep 20, 2007

Est. expiryMar 15, 2021(expired)· nominal 20-yr term from priority

Inventors:Eva Rojer

C07K 14/811C07K 2319/40C12N 15/62C07K 14/4731A61P 35/00C07K 2319/00

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References

Claims

Abstract

The present invention relates to a SCCA1/SCCA2 fusion protein; plasmid containing the same; antibodies of said fusion protein; methods for detecting said protein; methods for diagnosing the presence or absence of SCC by determining the presence of SCCA1/SCCA2 fusion protein.

Claims

exact text as granted — not AI-modified

1 . A fusion transcript consisting of a homologue cross-over between two different genes with more than 80% sequence homology in certain regions, in particular regions of cross-over.

2 . A fusion transcript according to claim 1 , wherein the two genes are the genes of SCCA1 and SCCA2.

3 . A full length fusion transcript protein between SCCA1 and SCCA2 having switched reactive site loops compared to basic promoter.

4 . A substantially full length fusion transcript protein between SCCA1 and SCCA2 having switched reactive site loops compared to basic promoter.

5 . A fusion protein according to claim 4 coded by one or more of exons 2-7 of SCCA1 gene fused to exon 8 of SCCA2 gene.

6 . A fusion protein according to claim 1 coded by exon 2-7 of SCCA1 gene fused to exon 8 of SCCA2 gene.

7 . A fusion protein according to claim 4 coded by one or more of exons 2-7 of SCCA2 gene fused to exon 8 of SCCA1 gene.

8 . A fusion protein according to claim 1 coded by exon 2-7 of SCCA2 gene fused to exon 8 of SCCA1 gene.

9 . A fusion protein according to claim 5 , wherein the protein sequence is (SEQ ID NO: 1)
MNSLSEANTK FMFDLFQQFR KSKENNIFYS PISITSALGM VLLGAKDNTA QQIKKVLHFD QVTENTTGKA ATYHVDRSGN VHHQFQKLLTE FNKSTDAYE LKIANKLFGE KTYLFLQEYL DAIKKFYQTS VESVDFANAP EESRKKINSW VESQTNEKIK NLIPEGNIGS NTTLVLVNAI YFKGQWEKKF NKEDTKEEKF WPNKNTYKSI QMMRQYTSFH FASLEDVQAK VLEIPYKGKD LSMIVLLPNE IDGLQKLEEK LTAEKLMEWT SLQNMRETCV DLHLPRFKME ESYDLKDTLR TMGMVNIFNG DADLSGMTWS HGLSVSKVLH KAFVEVTEEG VEAAAATAVV VVELSSPSTN EEFCCNHPFL FFIRQNKTNS ILFYGRFSSP

10 . A DNA sequence sequence coding for a fusion SCCA1/SCCA2 protein.

11 . A DNA sequence comprising the nucleotide sequence of exon 2-7 of SCCA1 fused to the nucleotide sequence of exon 8 of SCCA2.

12 . A DNA sequence according to claim 11 , wherein the nucleotide sequence is (SEQ ID NO: 11)

ATGAATTCAC TCAGTGAAGC CAACACCAAG TTCATGTTCG

ACCTGTTCCA ACAGTTCAGA AAATCAAAAG AGAACAACAT

CTTCTATTCC CCTATCAGCA TCACATCAGC ATTAGGGATG

GTCCTCTTAG GAGCCAAAGA CAACACTGCA CAACAGATTA

AGAAGGTTCT TCACTTTGAT CAAGTCACAG AGAACACCAC

AGGAAAAGCT GCAACATATC ATGTTGATAG GTCAGGAAAT

GTTCATCACC AGTTTCAAAA GCTTCTGACT GAATTCAACA

AATCCACTGA TGCATATGAG CTGAAGATCG CCAACAAGCT

CTTCGGAGAA AAAACGTATC TATTTTTACA GGAATATTTA

GATGCCATCA AGAAATTTTA CCAGACCAGT GTGGAATCTG

TTGATTTTGC AAATGCTCCA GAAGAAAGTC GAAAGAAGAT

TAACTCCTGG GTGGAAAGTC AAACGAATGA AAAAATTAAA

AACCTAATTC CTGAAGGTAA TATTGGCAGC AATACCACAT

TGGTTCTTGT GAACGCAATC TATTTCAAAG GGCAGTGGGA

GAAGAAATTT AATAAAGAAG ATACTAAAGA GGAAAAA TTT

TGGCCAAACA AGAATACATA CAAGTCCATA CAGATGATGA

GGCAATACAC ATCTTTTCAT TTTGCCTCGC TGGAGGATGT

ACAGGCCAAG GTCCTGGAAA TACCATACAA AGGCAAAGAT

CTAAGCATGA TTGTGTTGCT GCCAAATGAA ATCGATGGTC

TCCAGAAG CT TGAAGAGAAA CTCACTGCTG AGAAATTGAT

GGAATGGACA AGTTTGCAGA ATATGAGAGA GACATGTGTC

GATTTACACT TACCTCGGTT CAAAATGGAA GAGAGCTATG

ACCTCAAGGA CACGTTGAGA ACCATGGGAA TGGTGAATAT

CTTCAATGGG GATGCAGACC TCTCAGGCAT GACCTGGAGC

CACGGTCTCT CAGTATCTAA AGTCCTACAC AAGGCCTTTG

TGGAGGTCAC TGAGGAGGGA GTGGAAGCTG CAGCTGCCAC

CGCTGTAGTA GTAGTCGAAT TATCATCTCC TTCAACTAAT

GAAGAGTTCT GTTGTAATCA CCCTTTCCTA TTCTTCATAA

GGCAAAATAA GACCAACAGC ATCCTCTTCT ATGGCAGATT

CTCATCCCCA

13 . A plasmid comprising the nucleotide sequence corresponding to one or more of exons 2-7 of SCCA1 gene fused to exon 8 of SCCA2 gene.

14 . A plasmid comprising the nucleotide sequence corresponding to exons 2-7 of SCCA1 fused to the nucleotide sequence of exon 8 of SCCA2.

15 . A plasmid comprising the nucleotide sequence corresponding to one or more of exons 2-7 of SCCA2 gene fused to exon 8 of SCCA1 gene.

16 . A plasmid comprising the nucleotide sequence corresponding to exons 2-7 of SCCA2r gene fused to exon 8 of SCCA1 gene.

17 . A plasmid according to claim 13 , comprising the nucleotide sequence: of claim 12 ATGAATTCAC TCAGTGAAGC CAACACCAAG TTCATGTTCG ACCTGTTCCA ACAGTTCAGA AAATCAAAAG AGAACAACAT CTTCTATTCC CCTATCAGCA TCACATCAGC ATTAGGGATG GTCCTCTTAG GAGCCAAAGA CAACACTGCA CAACAGATTA AGAAGGTTCT TCACTTTGAT CAAGTCACAG AGAACACCAC AGGAAAAGCT GCAACATATC ATGTTGATAG GTCAGGAAAT GTTCATCACC AGTTTCAAAA GCTTCTGACT GAATTCAACA AATCCACTGA TGCATATGAG CTGAAGATCG CCAACAAGCT CTTCGGAGAA AAAACGTATC TATTTTTACA GGAATATTTA GATGCCATCA AGAAATTTTA CCAGACCAGT GTGGAATCTG TTGATTTTGC AAATGCTCCA GAAGAAAGTC GAAAGAAGAT TAACTCCTGG GTGGAAAGTC AAACGAATGA AAAAATTAAA AACCTAATTC CTGAAGGTAA TATTGGCAGC AATACCACAT TGGTTCTTGT GAACGCAATC TATTTCAAAG GGCAGTGGGA GAAGAAATTT AATAAAGAAG ATACTAAAGA GGAAAAATTTT TGGCCAAACA AGAATACATA CAAGTCCATA CAGATGATGA GGCAATACAC ATCTTTTCAT TTTGCCTCGC TGGAGGATGT ACAGGCCAAG GTCCTGGAAA TACCATACAA AGGCAAAGAT CTAAGCATGA TTGTGTTGCT GCCAAATGAA ATCGATGGTC TCCAGAAG CT TGAAGAGAAA CTCACTGCTG AGAAATTGAT GGAATGGACA AGTTTGCAGA ATATGAGAGA GACATGTGTC GATTTACACT TACCTCGGTT CAAAATGGAA GAGAGCTATG ACCTCAAGGA CACGTTGAGA ACCATGGGAA TGGTGAATAT CTTCAATGGG GATGCAGACC TCTCAGGCAT GACCTGGAGC CACGGTCTCT CAGTATCTAA AGTCCTACAC AAGGCCTTTG TGGAGGTCAC TGAGGAGGGA GTGGAAGCTG CAGCTGCCAC CGCTGTAGTA GTAGTCGAAT TATCATCTCC TTCAACTAAT GAAGAGTTCT GTTGTAATCA CCCTTTCCTA TTCTTCATAA GGCAAAATAA GACCAACAGC ATCCTCTTCT ATGGCAGATT CTCATCCCCA (SEQ ID NO: 11), and deposited at ECACC under deposition number ECACC 01031315.

18 . A protein expression system for production of SCCA1/SCCA2 fusion protein.

19 . A recombinant bacteria comprising a plasmid according to claim 13 .

20 . A recombinant bacteria comprising a plasmid according to claim 14 .

21 . A recombinant E. coli comprising a plasmid according to claim 13 .

22 . A recombinant E. coli comprising a plasmid according to claim 14 .

23 . A method for detecting the gene rearrangement forming the SCCA1/SCCA2 fusion protein using a cDNA cloning and sequencing analysis of tumor DNA.

24 . A method for detecting the gene rearrangement forming the SCCA2/SCCA1 fusion protein using a cDNA cloning and sequencing analysis of tumor DNA.

25 . A method for detecting the gene rearrangement forming the SCCA1/SCCA2 fusion protein using a Southern blot-technology applied on tumor DNA.

26 . A method for detecting the gene rearrangement forming the SCCA2/SCCA1 fusion protein using a Southern blot-technology applied on tumor DNA.

27 . A method for detecting the gene rearrangement forming the SCCA1/SCCA2 fusion protein using a PCR-analysis technology.

28 . A method for detecting the gene rearrangement forming the SCCA2/SCCA1 fusion protein using a PCR-analysis technology.

29 . A method for detecting the gene rearrangement forming the SCCA1/SCCA2 fusion protein using an amino acid sequencing technology.

30 . A method for detecting the gene rearrangement forming the SCCA2/SCCA1 fusion protein using an amino acid sequencing technology.

31 . A method for detection the SCCA1/A2 fusion protein using Western blotting.

32 . A method for detection the SCCA2/A1 fusion protein using Western blotting.

33 . A monoclonal antibody specific for SCCA1/SCCA2 fusion protein.

34 . A monoclonal antibody specific for SCCA2/SCCA2 fusion protein.

35 . A polyclonal antibody reactive with SCCA1/SCCA2 fusion protein.

36 . A monoclonal antibody specific for SCCA2/SCCA1 fusion protein.

37 . An immunoassay using a monoclonal antibody or polyclonal antibody specific
for SCCA1/SCCA2 fusion protein for detecting the presence and concentration of SCCA1/SCCA2 fusion protein.

38 . An immunoassay using a monoclonal antibody or polyclonal antibody specific for SCCA2/SCCA1 fusion protein for detecting the presence and concentration of SCCA2/SCCA1 fusion protein.

39 . A method for diagnosing the presence or absence of a squamous cell carcinoma by detecting the SCCA1/SCCA2 fusion protein in a human sample.

40 . A method for diagnosing the presence or absence of a squamous cell carcinoma by detecting the SCCA2/SCCA1 fusion protein in a human sample.

41 . A method according to claim 39 , wherein the fusion protein is used in a histochemical analysis.

42 . A kit comprising a SCCA1/SCCA2 fusion protein antibody to be used in the determination of the presence or absence of squamous cell carcinoma (SCC).

43 . A kit comprising a SCCA2/SCCA1 fusion protein antibody to be used in the determination of the presence or absence of squamous cell carcinoma (SCC).

44 . A kit according to claim 42 , in that it further comprises antibodies related to SCCA1 and/or SCCA2.

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