Biochemical analysis unit and method of producing thereof

Abstract

The biochemical analysis unit has a base plate and absorptive regions. The absorptive regions are surrounded by the base plate formed of materials which shield a radioactive ray and a light. In the absorptive regions are applied and absorbed specific binding substances to be bound with substances derived from a living organism that are labeled with labeling substances for generating the radioactive ray or the light. The base plate prevents the specific binding substances from penetrating in the other absorptive regions. When an analysis of data of the radioactive ray and the light is carried out, an image of the radioactive ray and the light is generated without noises.

1 . A biochemical analysis unit used for analyzing a data of a radioactive ray and a light, comprising: 
 an absorptive membrane formed of an absorptive material;    a shielding area formed in a surface of said absorptive membrane, containing metal colloid particles so as to shield a radioactive ray and a light; and    plural absorptive regions formed in said surface of said absorptive membrane, to said absorptive regions substances emitting at least one of said radioactive ray and said light being applied.    
     
     
         2 . A biochemical analysis unit as described in  claim 1 , wherein in said absorptive regions are absorbed specific binding substances which can specifically bind with substances derived from living organism that are labeled by at least one of labeling substances including radioactive labeling substances, fluorescent substances and chemiluminescent labeling substances.  
     
     
         3 . A biochemical analysis unit as described in  claim 2 , wherein said specific binding substances and said substances derived from living organism are bound through one of hybridization, antigen-antibody reaction and receptor-ligand.  
     
     
         4 . A biochemical analysis unit as described in  claim 2 , wherein the number of said absorptive regions is more than 10.  
     
     
         5 . A biochemical analysis unit as described in  claim 2 , wherein the averaged density of the number of said absorptive regions is more than 10/cm 2 .  
     
     
         6 . A biochemical analysis unit as described in  claim 2 , wherein said plural absorptive regions are formed in a regular pattern.  
     
     
         7 . A biochemical analysis unit as described in  claim 2 , wherein said absorptive material is a porous material.  
     
     
         8 . A biochemical analysis unit as described in  claim 2 , wherein said absorptive material contains a fiber material.  
     
     
         9 . A biochemical analysis unit for analyzing a data of a radioactive ray and a light, comprising: 
 a pair of plates members formed of a material which shields at least one of said radioactive ray and said light, said plate members having inner surfaces and outer surfaces respectively and superposing on each other with contact of said inner surfaces;    plural through-holes which are formed in said pair of said plate members;    absorptive material supplied in said plural through-holes; and    absorptive regions formed of said absorptive material in outer surfaces of said plate members, to said absorptive regions substances emitting at least one of said radioactive ray and said light being applied.    
     
     
         10 . A biochemical analysis unit as described in  claim 9 , wherein in said absorptive regions are absorbed specific binding substances which can specifically bind with substances derived from a living organism that are labeled by at least one of labeling substances including radioactive labeling substances, fluorescent substances and chemiluminescent labeling substances.  
     
     
         11 . A method of producing a biochemical analysis unit including a plate member and an absorptive membrane, said plate member having plural through-holes and being formed of a material decreasing at least one of a radioactive ray and a light, and said absorptive membrane being formed of an absorptive material, said method comprising steps of: 
 pressing said plate member onto said absorptive membrane such that a first surface of said plate member contacts to said absorptive membrane; and    supplying a part of said absorptive membrane in said through-holes by pressing said plate member to form absorptive regions in said through-holes in a second surface of said plate member for applying substances emitting at least one of said radioactive ray and said light.    
     
     
         12 . A method as described in  claim 11 , wherein said plural absorptive regions absorbs specific binding substances whose structures and characteristics are known, and said binding substances bind with substances derived from living organism that are labeled by at least labeling substances of radioactive labeling substances, fluorescent substances and chemiluminescent labeling substances.  
     
     
         13 . A method as described in  claim 12 , wherein said specific binding substances and said substances derived from living organism are bound through one of hybridization, antigen-antibody reaction and receptor-ligand.  
     
     
         14 . A method as described in  claim 13 , wherein said plate member is heated in the thermal press processing when pressed onto said absorptive membrane.  
     
     
         15 . A method as described in  claim 14 , wherein a calender-roller pair is used when said plate member is pressed onto said absorptive membrane.  
     
     
         16 . A method as described in  claim 15 , wherein said plate member is fixed to said absorptive membrane through an adhesive agent.  
     
     
         17 . A method as described in  claim 13 , wherein said plate member decreases a density of said radioactive ray and the light less than ⅕ when said radioactive ray and the light passes in said plate member for a length corresponding to a distance between the nearest two of said absorptive regions.  
     
     
         18 . A method as described in  claim 17 , wherein said plate member is formed of at least one of metallic materials, ceramic materials and plastic materials.  
     
     
         19 . A method as described in  claim 18 , wherein said plate member is formed of said plastic materials containing particles of oxides of metals.  
     
     
         20 . A method of producing a biochemical analysis unit having an absorptive membrane formed of absorptive material, comprising steps of: 
 covering parts of a surface of said absorptive membrane with a cover member;    supplying a solution containing metal colloid particles on other area than said parts so as to form a shielding area for shielding at least one of a radioactive ray and a light; and    removing said cover member from said parts of said surface to expose absorptive regions to which substances emitting at least one of said radioactive ray and said light are to be applied.    
     
     
         21 . A method as described in  claim 20 , wherein said plural absorptive regions absorbs specific binding substances whose structures and characteristics are known, and said binding substances bind with substances derived from living organism that are labeled by at least labeling substances of radioactive labeling substances, fluorescent substances and chemiluminescent labeling substances.  
     
     
         22 . A method as described in  claim 21 , wherein said specific binding substances and said substances derived from the living organism are bound through one of hybridization, antigen-antibody reaction and receptor-ligand.  
     
     
         23 . A method as described in  claim 21 , wherein the number of said absorptive regions is more than 10.  
     
     
         24 . A method as described in  claim 21 , wherein the size of an area in which said absorptive regions are formed is less than 5 mm 2 .  
     
     
         25 . A method as described in  claim 21 , wherein the density of the number of said absorptive regions is more than 10/cm 2 .  
     
     
         26 . A method as described in  claim 21 , wherein said absorptive regions are arranged in a regular pattern.  
     
     
         27 . A method as described in  claim 21 , wherein said absorptive material is a porous material.  
     
     
         28 . A method as described in  claim 27 , wherein said porous material is a carbon porous material or may be used for membrane filter.  
     
     
         29 . A method as described in  claim 21 , wherein said absorptive material contains a fiber material.  
     
     
         30 . A method as described in  claim 21 , wherein said biochemical analysis unit is superposed on a stimulable phosphor sheet on which a stimulable phosphor regions are formed, such that each of said stimulate phosphor region may be contact to said absorptive region so as to expose said stimulate phosphor region, and stimulate phosphors contained in said stimulate phosphor regions are excited to generate an emission light when an exciting light beam is applied on said stimulable phosphor region.  
     
     
         31 . A method of producing a biochemical analysis unit including a plate member and absorptive regions, said plate member being formed of a material which shields at least one of a radioactive ray and a light, said absorptive regions being formed of absorptive materials in two surfaces of said plate member, said method comprising steps of: 
 forming plural through-holes in said plate member; and    supplying said absorptive material in said plural through-holes so as to form in said through-holes said absorptive region to which substances emitting at least one of said radioactive ray and said light are to be applied.    
     
     
         32 . A method as described in  claim 31 , wherein said plural absorptive regions absorbs specific binding substances whose structures and characteristics are known, and said binding substances bind with substances derived from living organism that are labeled by at least labeling substances of radioactive labeling substances, fluorescent substances and chemiluminescent labeling substances.  
     
     
         33 . A method as described in  claim 32 , wherein said biochemical analysis unit is laid on a stimulable phosphor sheet on which a stimulable phosphor regions are formed, such that each of said stimulate phosphor region may be contact to said absorptive region so as to expose said stimulate phosphor region, and stimulate phosphors contained in said stimulate phosphor regions are excited to generate an emission light when an exciting light beam is applied on said stimulable phosphor region.  
     
     
         34 . A method as described in  claim 32 , wherein said absorptive material contains a little of bad solvent and a main component of good solvent, further comprising steps of: 
 setting said plate member in a solidifying solution after providing said absorptive material in said through-holes; and    cleaning out said solidifying solution in water.    
     
     
         35 . A method as described in  claim 32 , wherein said absorptive material is provided on a surface of said plate member so as to cover said surface, further comprising steps of: 
 pressing said absorptive material and said plate member so as to supply a part of said absorptive material in said through-hole; and    removing another part of said absorptive material from said surface of said plate member.    
     
     
         36 . A method as described in  claim 32 , wherein said absorptive material is a porous material.  
     
     
         37 . A method as described in  claim 36 , wherein said porous material is used for forming a membrane filter.  
     
     
         38 . A method as described in  claim 32 , wherein a fiber material which does not solve in a solvent of said porous material is mixed in said porous material.  
     
     
         39 . A method as described in  claim 32 , wherein said plate member decreases a density of said radioactive ray and the light less than ⅕ when said radioactive ray and the light passes in said plate member at a length corresponding to a distance between the nearest two of said through-holes.  
     
     
         40 . A method as claimed in  claim 32 , wherein said plate member is formed of metallic materials, ceramic materials and plastic materials.  
     
     
         41 . A method as claimed in  claim 32 , wherein said through-holes are formed with a punch.  
     
     
         42 . A method as claimed in  claim 32 , wherein said through-holes are formed with an electric discharging machine.  
     
     
         43 . A method as claimed in  claim 32 , wherein said through-holes are formed through photo lithograph and etching.  
     
     
         44 . A method as claimed in  claim 32 , wherein said through-holes are formed through razor ablation.  
     
     
         45 . A method as claimed in  claim 32 , wherein the size of each of said through-holes is less than 5 mm 2 .  
     
     
         46 . A method as claimed in  claim 32 , wherein the density of the number of said through-holes is more than 10/mm 2 .  
     
     
         47 . A method of producing a biochemical analysis unit including a plate member and absorptive regions, said plate member being formed of a material which shields at least one of a radioactive ray and a light, said absorptive regions being formed of absorptive materials, said method comprising steps of: 
 forming plural through-holes in said plate member;    supplying for said through-holes a solution or a diffusing solution of said porous material;    drying a solvent of said solution or said diffusing solution to form said absorptive regions in said through-holes in two surfaces of said plate member for applying substances emitting at least one of said radioactive ray and said light.    
     
     
         48 . A method as claimed in  claim 47 , wherein said solution or said diffusing solution is provided in an anti-solvent of said porous material.  
     
     
         49 . A method as claimed in  claim 48 , wherein said porous material is composed of organic high molecular substances and covers as a membrane a wall surrounding each of said through-holes, and extremely small holes are formed in said membrane.  
     
     
         50 . A method as claimed in  claim 49 , wherein said plate member is formed of at least one of metal material, plastic material and ceramics.  
     
     
         51 . A method as claimed in  claim 50 , wherein the averaged density of said plate member is more than 0.6 g/cm 3 , and the averaged density of said porous material is less than 1.0 g/cm 3 .  
     
     
         52 . A method as claimed in  claim 51 , wherein said through-holes are arranged in a pitch of 0.1-3 mm.  
     
     
         53 . A method as claimed in  claim 51 , wherein said through-holes are arranged with a distance between 0.05 and 1.5 mm.  
     
     
         54 . A method as claimed in  claim 51 , wherein said absorptive regions are retracted from one or both of surfaces of said plate member.  
     
     
         55 . A method of producing a biochemical analysis unit including two plate members and absorptive regions, said two plate members being formed of a material which shields at least one of a radioactive ray and a light, said absorptive regions being formed of absorptive materials, said method comprising steps of: 
 forming through-holes in each of said two plate members;    disposing a porous material sheet made of porous materials between said two plate members;    pressing said two plate members to each other to sandwich said porous material sheet; and    providing a part of said porous material sheet into said through-holes by pressing said two plate members so as to form said absorptive regions in said through-holes in an outer surface of each of said plate members for applying substances emitting at least one of said radioactive ray and said light.    
     
     
         56 . A method as claimed in  claim 55 , wherein said two plate members are heated when pressed to each other.  
     
     
         57 . A method as claimed in  claim 55 , wherein said two plate members are formed of metal materials or ceramic materials.  
     
     
         58 . A method as claimed in  claim 55 , wherein a solvent is used for solving said two plate members without solving said porous material sheet when the two plate members are pressed to each other.  
     
     
         59 . A method as claimed in  claim 58 , wherein said two plate members are formed of plastic materials.  
     
     
         60 . A method as claimed in  claim 55 , wherein said porous material is organic high molecular material.  
     
     
         61 . A method as claimed in  claim 55 , wherein the averaged density of said plate member is more than 0.6 g/cm 3 , and the averaged density of said porous material is less than 1.0 g/cm 3 .  
     
     
         62 . A method as claimed in  claim 61 , wherein said through-holes are arranged in a pitch of 0.1-3 mm.  
     
     
         63 . A method as claimed in  claim 61 , wherein said through-holes are arranged with a distance between 0.05 and 1.5 mm.  
     
     
         64 . A method as claimed in  claim 61 , wherein said absorptive regions are retracted from one or both of surfaces of said plate member.