Affinity particle and affinity separation method

Abstract

The present invention is affinity particles that are characterized by having phosphorylcholine groups represented by the following formula (1) covalently bonded onto the surface of inorganic powder and also by having ligands having specific affinity with a certain target substance covalently bonded or adsorbed onto the surface of inorganic powder. The object of the present invention is to provide an affinity separation method that uses affinity particles utilizing inexpensive inorganic particles and is capable of separating the target substance easily and with high accuracy.

1 . Affinity particles comprising phosphorylcholine groups represented by the following formula (1) covalently bonded onto the surface of inorganic particles.  
     
       
         
         
             
             
         
       
     
   
   
       2 . Affinity particles comprising phosphorylcholine groups represented by the following formula (1) covalently bonded onto the surface of inorganic particles and also by having reactive groups or adsorptive groups, which are capable of bonding with ligands having specific affinity with a certain target substance, covalently bonded or adsorbed onto the surface of inorganic particles.  
     
       
         
         
             
             
         
       
     
   
   
       3 . Affinity particles comprising phosphorylcholine groups represented by the following formula (1) covalently bonded onto the surface of inorganic particles and also by having ligands having specific affinity with a certain target substance covalently bonded or adsorbed onto the surface of inorganic particles.  
     
       
         
         
             
             
         
       
     
   
   
       4 . The affinity particles of  claim 1 , wherein said inorganic particles are selected from a group consisting of silica, titanium oxide, zinc flower, alumina, iron oxide, talc, mica, sericite, and gold colloid, and having an average particle size of 20 nm to 500 μm and a specific gravity of 1.0 g/cm 3  or higher.  
   
   
       5 . The affinity particles of  claim 1 , wherein said ligands are one, two, or more types of ligands chosen from a group consisting of various antibodies, antigens, enzymes, substrates, receptors, lectin, peptides, DNA, RNA, aptamers, protein A, protein G, avidin, biotin, chelating compounds, and various metal ions.  
   
   
       6 . A method of affinity separation of a target substance by using inorganic particles comprising: 
 (1) a first process whereby arbitrary ligands are bonded to the affinity particles of  claim 1 ,    (2) a second process whereby the affinity particles prepared in the first process are dispersed in a solution sample containing a target substance selectively captured by the arbitrary ligands, and    (3) a third process whereby the target substance captured is recovered from the affinity particles.    
   
   
       7 . A method of affinity separation of a target substance by using inorganic particles comprising: 
 (1) a first process whereby the affinity particles of  claim 3  are dispersed in a solution sample containing a target substance selectively captured by the arbitrary ligands, and    (2) a second process whereby the target substance captured is recovered from the affinity particles.    
   
   
       8 . The affinity particles of  claim 2 , wherein said inorganic particles are selected from a group consisting of silica, titanium oxide, zinc flower, alumina, iron oxide, talc, mica, sericite, and gold colloid, and having an average particle size of 20 nm to 500 μm and a specific gravity of 1.0 g/cm 3  or higher.  
   
   
       9 . The affinity particles of  claim 3 , wherein said inorganic particles are selected from a group consisting of silica, titanium oxide, zinc flower, alumina, iron oxide, talc, mica, sericite, and gold colloid, and having an average particle size of 20 nm to 500 μm and a specific gravity of 1.0 g/cm 3  or higher.  
   
   
       10 . The affinity particles of  claim 2 , wherein said ligands are one, two, or more types of ligands chosen from a group consisting of various antibodies, antigens, enzymes, substrates, receptors, lectin, peptides, DNA, RNA, aptamers, protein A, protein G, avidin, biotin, chelating compounds, and various metal ions.  
   
   
       11 . The affinity particles of  claim 3 , wherein said ligands are one, two, or more types of ligands chosen from a group consisting of various antibodies, antigens, enzymes, substrates, receptors, lectin, peptides, DNA, RNA, aptamers, protein A, protein G, avidin, biotin, chelating compounds, and various metal ions.  
   
   
       12 . The affinity particles of  claim 4 , wherein said ligands are one, two, or more types of ligands chosen from a group consisting of various antibodies, antigens, enzymes, substrates, receptors, lectin, peptides, DNA, RNA, aptamers, protein A, protein G, avidin, biotin, chelating compounds, and various metal ions.  
   
   
       13 . A method of affinity separation of a target substance by using inorganic particles comprising: 
 (1) a first process whereby arbitrary ligands are bonded to the affinity particles of  claim 2 ,    (2) a second process whereby the affinity particles prepared in the first process are dispersed in a solution sample containing a target substance selectively captured by the arbitrary ligands, and    (3) a third process whereby the target substance captured is recovered from the affinity particles.