Method of Drug Interaction

Abstract

A method of interacting a drug with an in vivo system having multiple cell types to model a drug interaction with a living organism in a cell culture tool having a flat plate segregated into multiple chamber units, each of the chamber units having a chamber wall surrounding multiple wells with the height of the chamber wall being taller than that of the height of the surrounded multiple wells, including depositing a different cell type in the set of wells in each chamber, adding a fluid medium to a height above the flat surface to fluidly interconnect the set of wells in each chamber and adding the drug to the fluid medium in each chamber, the dose and type of the drug being varied in each chamber.

1 . A method of interacting a drug with more than one type of cell material in a culture dish having a flat surface with a plurality of depressions defining wells, and a containing wall surrounding the flat surface, the method comprising: 
 depositing different cell types in separate wells of the culture dish;    adding a fluid medium to a height above the flat surface to fluidly interconnect the wells; and    adding the drug to the fluid medium.    
     
     
         2 . The method of  claim 1 , further comprising: 
 adding one or more culture medium to each of the separate wells; and    culturing more than the different cell types in the separate wells.    
     
     
         3 . The method of  claim 2 , wherein one of the different cell types comprises liver cells.  
     
     
         4 . The method of  claim 2 , wherein one of the different cell types comprises kidney cells.  
     
     
         5 . The method of  claim 2 , wherein one of the different cell types comprises lung cells.  
     
     
         6 . The method of  claim 2 , wherein one of the different cell types comprises spleen cells.  
     
     
         7 . The method of  claim 2 , wherein depositing different cell types comprises depositing tissue fragments or fractions that include the different cell types.  
     
     
         8 . The method of  claim 2 , wherein depositing different cell types comprises 
 depositing 
 kidney cells in a first well of the wells,  
 liver cells in a second well of the wells,  
 heart cells in a third well of the wells,  
 lung cells in a fourth well of the wells,  
 spleen cells in a fifth well of the wells, and  
 brain cells in a sixth well of the six wells.  
   
     
     
         9 . The method of  claim 2 , further comprising: 
 metabolizing the drug by the different cell types.    
     
     
         10 . A method of metabolizing a drug in a cell culture tray having six wells surrounded by a containing wall, the method comprising: 
 depositing a first cell type in a first well, a second cell type in a second well, a third cell type in a third well, a fourth cell type in a fourth well, a fifth cell type in a fifth well, and a sixth cell type in a sixth well;    overfilling the first, second, third, fourth, fifth, and sixth well to cause the first, second, third, fourth, fifth, and sixth cell type to interact via a common fluid medium; and    introducing the drug into the common fluid medium.    
     
     
         11 . The method of  claim 10 , wherein the first cell type comprises liver cells, the second cell type comprises kidney cells, the third cell type comprises heart cells, the fourth cell type comprises spleen cells, the fifth cell type comprises brain cells, and the sixth cell type comprises lung cells.  
     
     
         12 . The method of  claim 10 , wherein the first, second and third cell type comprise liver cells and the fourth, fifth, and sixth cell type comprise heart cells.  
     
     
         13 . A method of interacting a drug with an in vivo system having multiple cell types to model a drug interaction with a living organism in a cell culture tool having a flat plate segregated into multiple chamber units, each of the chamber units having a chamber wall surrounding multiple wells with the height of the chamber wall being taller than that of the height of the surrounded multiple wells, the method comprising: 
 depositing a different cell type in the set of wells in each chamber;    adding a fluid medium to a height above the flat surface to fluidly interconnect the set of wells in each chamber; and    adding the drug to the fluid medium in each chamber, the dose and type of the drug being varied in each chamber.    
     
     
         14 . The method of  claim 13 , further comprising: 
 providing the tool having a plate segregated into  12  chamber units each having a chamber wall surrounding  8  of the wells.    
     
     
         15 . The method of  claim 13 , further comprising: 
 providing the tool having a plate segregated into  64  chamber units each having a chamber wall surrounding  6  of the wells.    
     
     
         16 . The method of  claim 13 , further comprising: 
 providing the tool having a plate segregated into  256  chamber units each having a chamber wall surrounding  6  of the wells.