US2007161083A1PendingUtilityA1
Codon Optimized Recombinant Dermaphagoides Allergens
Est. expiryJun 10, 2020(expired)· nominal 20-yr term from priority
A61P 37/08A61K 38/00A61P 11/06C07K 14/43531
50
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Claims
Abstract
The present invention relates to codon optimised polynucleotides which are efficiently expressed in mammalian cells and encode insect proteins from Dermaphagoides dust mite. In particular, the optimised codon polynucleotides encode a protein from Dermaphagoides pteronyssinus , such as DerP1 or proDerP1. The present invention also provides methods of preparing pharmaceutical compositions comprising the expression of the codon optimised polynucleotides, and vectors and transformed host cells comprising them.
Claims
exact text as granted — not AI-modified1 . An isolated polynucleotide sequence which encodes a Dermatophagoides mite protein, wherein the codon usage pattern of the polynucleotide sequence resembles that of highly expressed mammalian genes.
2 . An isolated polynucleic acid sequence as claimed in claim 1 , wherein all of the amino acid types present in the protein are optimised, such that the codons used to encode that amino acid are used in the same frequency as the known mammalian frequency for that amino acid.
3 . An isolated polynucleic acid sequence as claimed in claim 1 , wherein the yield of protein encoded by the polynucleic acid when expressed by an expression system is greater than 20% higher than the amount of protein produced from the same expression system using a non-optimised native gene for that Dermatophagoides protein.
4 . An isolated polynucleic acid sequence encoding a Dermaphagoides protein, characterised in that the codons present in said polynucleotide which are used to encode each amino acid are selected to appear as set forth in the following table
Amino
Frequency
Acid
Codon
(percentage used)
Ala
GCG
17
GCA
13
GCT
17
GCC
53
Arg
AGG
18
AGA
10
CGG
21
CGA
6
CGT
7
CGC
37
Asn
AAT
22
AAC
78
Asp
GAT
25
GAC
75
Cys
TGT
32
TGC
68
Gln
CAG
88
CAA
12
Glu
GAG
75
GAA
25
Gly
GGG
24
GGA
14
GGT
12
GGC
50
His
CAT
21
CAC
79
Ile
ATA
5
ATT
18
ATC
77
Leu
TTG
6
TTA
2
CTG
58
CTA
3
CTT
5
CTC
26
Lys
AAG
82
AAA
18
Phe
TTT
20
TTC
80
Pro
CCG
17
CCA
16
CCT
19
CCC
48
Ser
AGT
10
AGC
34
TCG
9
TCA
5
TCT
13
TCC
28
Thr
ACG
15
ACA
14
ACT
14
ACC
57
Tyr
TAT
26
TAC
74
Val
GTG
64
GTA
5
GTT
7
GTC
25
5 . An isolated polynucleotide comprising SEQ ID NO: 15.
6 . An isolated vector comprising the isolated polynucleotide of claim 5 .
7 . An isolated host cell comprising the vector of claim 6.Join the waitlist — get patent alerts
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