Composition for prevention and treatment of diabetic complication

Abstract

The present invention relates to a composition for the prevention and treatment of diabetic complications, which contains an extract obtained by: crushing and drying any one selected from Euphorbiae radix, gingered Magnolia bark, parched Puerariae radix and Glycyrrhizae radix; extracting the dried herbal material with alcohol or aqueous alcohol solution: filtering the extract; and concentrating the filtrate under reduced pressure. Alternatively, the composition contains a mixture of extracts from Euphorbiae radix, gingered Magnolia bark, parched Puerariae radix and Glycyrrhizae radix, the amount of each of the herbal extracts being 5-85% by weight based on 100% by weight of the herbal extract mixture. Alternatively, the composition contains magnolol obtained by: extracting Magnolia bark with aqueous 80% ethanol solution at ambient temperature for 24 hours; systematically separating the extract in the order of normal-hexane, ethyl acetate and normal-butanol; selecting the normal-hexane layer and separating the selected layer into pre-fractions by normal silica gel chromatography; comparing the pre-fractions with the standard form magnolol on TLC to determine a magnolol-enriched fraction; and separating magnolol from the magnolol-enriched fraction by silica gel column chromatography.

1 . A composition for the prevention and treatment of diabetic complications, which contains an extract obtained by: crushing and drying anyone selected from  Euphorbiae  radix, gingered  Magnolia  bark, parched  Puerariae  radix and  Glycyrrhizae  radix; extracting the dried herbal material with alcohol or aqueous alcohol solution; filtering the extract; and concentrating the filtrate under reduced pressure.  
   
   
       2 . A composition for the prevention and treatment of diabetic complications, which contains a mixture of extracts from  Euphorbiae  radix, gingered  Magnolia  bark, parched  Puerariae  radix and  Glycyrrhizae  radix, the amount of each of the herbal extracts being 5-85% by weight based on 100% by weight of the herbal extract mixture.  
   
   
       3 . A composition for the prevention and treatment of diabetic complications, which contains an herbal extract mixture obtained by: crushing and drying each of  Euphorbiae  Radix, gingered  Magnolia  bark, parched  Puerariae  radix and  Glycyrrhizae  radix: mixing the crushed herbal materials together, each of the herbal materials being used at an amount of 5-85% by weight based on the total weight of the herbal materials taken as 100% by weight: extracting the herbal mixture with alcohol or aqueous alcohol solution; filtering the extract; and concentrating the filtrate under reduced pressure.  
   
   
       4 . A pharmaceutical composition for the prevention or treatment of diabetic complications, which contains, as an active ingredient, an extract obtained as claimed in  claim 1 .  
   
   
       5 . A functional food for the prevention or treatment of diabetic complications, which contains, as an active ingredient, an extract obtained as claimed in  claim 1 .  
   
   
       6 . A pharmaceutical composition for the prevention and delay of aging, which contains, as an active ingredient, an extract obtained as claimed in  claim 1 .  
   
   
       7 . A functional food for the prevention and delay of aging, which contains, as an active ingredient, an extract obtained as claimed in  claim 1 .  
   
   
       8 . A composition for the prevention or treatment of diabetic complications, which contains, as an active ingredient, magnolol isolated from  Magnolia  bark, or a pharmaceutically acceptable salt thereof.  
   
   
       9 . The composition of  claim 8 , wherein the magnolol is obtained by: extracting  Magnolia  bark with 80% ethanol aqueous solution at ambient temperature for 24 hours; systematically separating the extract in the order of normal-hexane, ethyl acetate and normal-butanol; selecting the normal-hexane layer 20 and separating the selected layer into pre-fractions by normal silica gel chromatography; comparing the pre-fractions with the standard form magnolol on TLC to determine a magnolol-enriched fraction; and separating magnolol from the magnolol-enriched fraction by silica gel column chromatography.  
   
   
       10 . A pharmaceutical composition for the prevention or treatment of diabetic complications, which contains, as an active ingredient, magnolol obtained as claimed in  claim 8 .  
   
   
       11 . A functional food for the prevention or treatment of diabetic complications, which contains, as an active ingredient, magnolol obtained as claimed in  claim 8 .  
   
   
       12 . A pharmaceutical composition for the prevention and 35 delay of aging, which contains, as an active ingredient, magnolol obtained as claimed in  claim 8 .  
   
   
       13 . A functional food for the prevention and delay of aging, which contains, as an active ingredient, magnolol obtained as claimed in  claim 8.