Fungal Resistant Transgenic Plants

Abstract

Transgenic plants that are resistant to Sclerotinia and Phoma lingam are described as well as methods for producing such transgenic plants.

1 . A method of improving  Phoma lingam  resistance in a  Brassica  plant line said method comprising 
 a) introducing at least one nucleic acid construct into cells of a  Brassica  species that is resistant to at least one strain of  Phoma lingam , wherein said nucleic acid construct comprises i) a regulatory element operably linked to a nucleic acid encoding an osmotin polypeptide; ii) a regulatory element operably linked to a nucleic acid encoding a chitinase polypeptide; iii) a regulatory element operably linked to a nucleic acid encoding a β 1,3 glucanase polypeptide; and iv) a regulatory element operably linked to a nucleic acid encoding a PR1 polypeptide;    b) obtaining one or more progeny plants from said cells;    c) identifying at least one of said progeny plants that has improved resistance to said at least one strain of  Phoma lingam ; and    d) producing said plant line from said at least one progeny plant by self- or cross-pollination, wherein said plant line has improved resistance to said at least one strain of  Phoma lingam.      
   
   
       2 . A method of producing a plant line resistant to  S. sclerotiorum , said method comprising 
 a) introducing at least one nucleic acid construct into cells of a plant species that is susceptible to  S. sclerotiorum , wherein said nucleic acid construct comprises i) a regulatory element operably linked to a nucleic acid encoding a first chitinase polypeptide; ii) a regulatory element operably linked to a nucleic acid encoding a second chitinase polypeptide; iii) a regulatory element operably linked to a nucleic acid encoding a β-1,3-glucanase polypeptide; iv) a regulatory element operably linked to a nucleic acid encoding an osmotin polypeptide; and v) a regulatory element operably linked to a nucleic acid encoding a PR1 polypeptide;    b) obtaining one or more progeny plants from said cells;    c) identifying at least one of said progeny plants that is resistant to  S. sclerotiorum ; and    d) producing said plant line from said at least one progeny plant by self- or cross-pollination, wherein said plant line is resistant to  S. sclerotiorum.      
   
   
       3 . A method of producing a plant line resistant to  S. sclerotiorum , said method comprising 
 a) introducing at least one nucleic acid construct into cells of a plant species that is susceptible to  S. sclerotiorum , wherein said nucleic acid construct comprises i) a regulatory element operably linked to a nucleic acid encoding a chitinase polypeptide; ii) a regulatory element operably linked to a nucleic acid encoding a first β-1,3-glucanase polypeptide; iii) a regulatory element operably linked to a nucleic acid encoding a second β-1,3-glucanase polypeptide; iv) a regulatory element operably linked to a nucleic acid encoding an osmotin polypeptide; and v) a regulatory element operably linked to a nucleic acid encoding a PR1 polypeptide;    b) obtaining one or more progeny plants from said cells;    c) identifying at least one of said progeny plants that is resistant to  S. sclerotiorum ; and    d) producing said plant line from said at least one progeny plant by self- or cross-pollination, wherein said plant line is resistant to  S. sclerotiorum.