Method for determining botulinum potency

Abstract

Skin topographical methods for quantifying pharmacodynamic parameters of a paralytic effect of a Clostridial toxin, such as a botulinum toxin upon a muscle, such as a frontalis muscle. The methods are effective in determining the potency of a Clostridial toxin, and in comparing toxins. For example, wrinkle reduction effects caused by one or more neurotoxins may be monitored, and differences in effects correspond to differences of neurotoxins.

1 - 15 . (canceled)  
     
     
         16 . A method for determining the potency of a botulinum neurotoxin, comprising the steps of: examining a muscle of an individual at a maximum voluntary contraction; administering a composition comprising a botulinum neurotoxin to the muscle; and examining the muscle after the muscle has been administered the botulinum neurotoxin without killing the individual to determine an effect of the botulinum neurotoxin on the muscle.  
     
     
         17 . The method of  claim 16 , further comprising repeating the steps for a plurality of individuals.  
     
     
         18 . The method of  claim 17 , further comprising analyzing the effect of the botulinum neurotoxin on the muscle as a function of amount of the botulinum neurotoxin in the composition for each individual administered the composition.  
     
     
         19 - 42 . (canceled)