Human HAC3

Abstract

The invention provides isolated nucleic acid and amino acid sequences of hHAC3, antibodies to hHAC3, methods of detecting hHAC3, and methods of screening for modulators hyperpolarization-activated cation channels using biologically active hHAC3. The invention further provides, in a computer system, a method of screening for mutations of human HAC3 genes as well as a method for identifying a three-dimensional structure of human HAC3 polypeptides.

1 - 19 . (canceled)  
     
     
         20 . An antibody that selectively binds to an isolated polypeptide comprising an alpha subunit of a cation channel, the polypeptide: 
 (i) forming, with at least one additional HAC alpha subunit, a cation channel having the characteristic of activation upon hyperpolarization; and    (ii) having an amino acid sequence that has greater than about 96% identity to SEQ ID NO:1.    
     
     
         21 . The antibody of  claim 20 , wherein the polypeptide has an amino acid sequence of SEQ ID NO:1.  
     
     
         22 - 39 . (canceled)  
     
     
         40 . The antibody of  claim 20 , wherein the polypeptide comprises an alpha subunit of a homomeric cation channel.  
     
     
         41 . The antibody of  claim 20 , wherein the polypeptide comprises an alpha subunit of a heteromeric cation channel.  
     
     
         42 . The antibody of  claim 20 , wherein the antibody is a monoclonal antibody.