Methods and compositions for controlling biofilm development

Abstract

A method of cleaning or protecting surfaces by treatment with compositions comprising N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL) blocking compounds and/or N-butyryl-L-homoserine lactone (BHL) analogs, either in combination or separately.

1 . A biofilm removing or inhibiting composition comprising an amount of a compound selected from the group consisting blockers of N-(3-oxododecanoyl) L-homoserine lactone (OdDHL), and analogs of butyryl L-homo-serine lactone (BHL), and mixtures thereof, and a vehicle or carrier, wherein the amount of the compound is effective to remove or disrupt a bacterial biofilm or inhibit normal biofilm formation.  
   
   
       2 . The composition of  claim 1  wherein the compound is an OdDHL blocker having the following structure:  
     
       
         
         
             
             
         
       
       wherein in the above formulae R 1 -R 2 , are selected from H, C 1 -C 4  alkyl group (preferably CH 3 ), OH, NH 2 , SH or a halogen such as fluorine, chlorine bromine or iodine;  
       R 22  and R 23  are selected from S and O,  
       R 24 -R 28  is H or a halogen, and  
       X, X 1  and X 2  are selected from O, S, H 2  or any combination of H plus one halogen or two halogens when one or more R groups is substituted.  
     
   
   
       3 . The composition in  claim 2  wherein R 22  through R 28  is a H or halogen.  
   
   
       4 . The composition in  claim 2  wherein one or more carbons forming the backbone of the molecule are substituted with S or S-substituted moieties.  
   
   
       5 . The composition of  claim 3  or  4  wherein the carbonyl group at X 1  and/or X 2  is substituted with H 2 , H plus a halogen or two halogens.  
   
   
       6 . The composition of  claim 1  wherein the compound is a butyryl-L-homoserine lactone (BHL) analog having the following structure:  
     
       
         
         
             
             
         
       
       wherein in the above formulae R 1 -R 2 , are selected from H, C 1 -C 4  alkyl group (preferably CH 3 ), OH, NH 2 , SH or a halogen such as fluorine, chlorine bromine or iodine;  
       R 22  and R 23  are selected from S and O,  
       R 24 -R 28  is H or a halogen, and  
       X, X 1  and X 2  are selected from O, S, H 2  or any combination of H plus one halogen or two halogens when one or more R groups is substituted.  
     
   
   
       7 . The composition of  claim 6 , wherein R 22  is selected from H, S, O and NH and R 23  is selected from S, O, and N.  
   
   
       8 . The composition of  claim 6 , wherein the acyl side chain contains one or more double bonds or triple bonds between carbon atoms within the acyl side chain.  
   
   
       9 . The composition of  claim 6  wherein X is selected from H 2 , H plus a halogen, two halogens, H plus OH or NH 2 , a double bonded O, NH or S.  
   
   
       10 . The composition of  claim 1  additionally comprising a surfactant selected from the group consisting of anionic, nonionic, amphoteric, biological surfactants and mixtures thereof.  
   
   
       11 . The composition of  claim 10 , wherein the surfactant is sodium dodecyl sulfate.  
   
   
       12 . The composition of  claim 2  in which the blocker is N-(2,2 difluorodecanoyl)-L-homoserine lactone.  
   
   
       13 . The composition of  claim 1  further comprising a compound selected from the group consisting of biocides, fungicides, antibiotics, and mixtures thereof.  
   
   
       14 . A method of removing biofilm from a surface comprising the step of administering a cleaning-effective amount of the composition of  claim 1  to a biofilm-containing surface.  
   
   
       15 . A method of preventing biofilm formation on a surface comprising the step of administering an effective amount of the composition of  claim 1  to a surface, wherein the amount is effective to prevent biofilm formation.  
   
   
       16 . The method of  claim 14  wherein the surface is a hard, rigid surface.  
   
   
       17 . The method of  claim 16  wherein the surface is selected from the group consisting of a drainpipe, glaze ceramic, porcelain, glass, metal, wood, chrome, plastic, vinyl, and formica.  
   
   
       18 . The method of  claim 14  wherein the surface is a soft, flexible surface.  
   
   
       19 . The method of  claim 18  wherein the surface is selected from the group consisting of shower curtains or liners, upholstery, laundry; and carpeting.  
   
   
       20 . The method of  claim 14  wherein the biofilm is produced by a bacteria of the class Pseudomonas.  
   
   
       21 . The method of  claim 20  wherein the bacteria is of the species  Pseudomonas aeuroginosa.    
   
   
       22 . The method of  claim 14  wherein the biofilm is produced by an organism selected from the group consisting of bacteria, algae, fungi and protozoa.  
   
   
       23 . A dentifrice comprising an effective amount of a composition of  claim 1 , wherein the amount is effective to either prevent or remove biofilm formation.  
   
   
       24 . A mouthwash comprising an effective amount of a composition of  claim 1 , wherein the amount is effective to either prevent or remove biofilm formation.  
   
   
       25 . A method for treatment of dental caries comprising administering an effective amount of a composition of  claim 1 , wherein the amount is effective to either prevent or remove biofilm formation.  
   
   
       26 . A method of prevention of dental caries comprising administering an effective amount of a composition of  claim 1 , wherein the amount is effective to either prevent or remove biofilm formation.  
   
   
       27 . A method of treatment of acne comprising topically administering an effective amount of a composition of  claim 1 , wherein the amount is effective to either prevent or remove biofilm formation.  
   
   
       28 . A method of cleaning and disinfecting contact lenses comprising administering an effective amount of a composition of  claim 1 , wherein the amount is effective to either prevent or remove biofilm formation.  
   
   
       29 . A microbial dispersing composition comprising an effective amount of composition of  claim 1 , wherein the amount is effective to either prevent or remove biofilm formation.