Methods for producing ligand arrays

Abstract

Methods for producing ligand arrays, e.g., peptide and nucleic acid arrays, as well as the arrays produced thereby, methods for use of the arrays and kits that include the same are provided. In one embodiment of the subject methods, a substrate having a surface displaying acetate functional groups that produce surface bound hydroxyl functional groups upon hydrolysis is first provided, where the acetate groups are then hydrolyzed to hydroxyl functional groups. In another embodiment of the subject methods, a substrate having a surface displaying alkenyl functional groups is first provided, where the alkenyl functional groups are then converted to carboxylic functional groups. The resultant substrates, optionally after an additional functionalization step, are then contacted with ligands, e.g., via deposition of each different ligand onto a different region of the surface, resulting in covalent attachment of the contacted ligands to the surface. The subject methods find use in the preparation of a variety of different types of arrays, where the produced arrays find use in a variety of different applications, including both genomic and proteomic applications.

1 - 12 . (canceled)  
     
     
         13 . A ligand array produced according to the method of  claim 36 .  
     
     
         14 - 27 . (canceled)  
     
     
         28 . A method of detecting the presence of an analyte in a sample, said method comprising: 
 (a) contacting a sample suspected of comprising said analyte with a ligand array according to  claim 13;     (b) detecting any binding complexes on the surface of said array to obtain binding complex data; and    (c) determining the presence of said analyte in said sample using said binding complex data.    
     
     
         29 . The method according to  claim 28 , wherein said analyte is a nucleic acid.  
     
     
         30 . The method according to  claim 28 , wherein said method further comprises a data transmission step in which a result from a reading of said array is transmitted from a first location to a second location.  
     
     
         31 . A method according to  claim 30 , wherein said second location is a remote location.  
     
     
         32 . A method comprising receiving data representing a result of a reading obtained by the method of  claim 30 .  
     
     
         33 . A kit for use in an analyte detection assay, said kit comprising: 
 a ligand array according to  claim 13 .    
     
     
         34 . A method of producing a surface modified substrate, said method comprising: 
 (a) contacting a surface of a substrate with a silanizing reagent comprising an acetate functional group to produce a surface displaying acetate functional groups;    (b) converting said acetate functional groups on said surface to hydroxyl functional groups to produce said surface modified substrate.    
     
     
         35 . A method of producing a surface modified substrate, said method comprising: 
 (a) contacting a surface of a substrate with a first silanizing reagent comprising an alkene functional group to produce a surface displaying alkene functional groups;    (b) converting said alkene functional groups on said surface to carboxylic functional groups to produce said surface modified substrate.    
     
     
         36 . A method of producing an array of at least two different ligands covalently bonded to a surface of a substrate, said method comprising: 
 (a) contacting a surface of a substrate with a silanizing reagent comprising an acetate functional group to produce a surface displaying acetate functional groups;    (b) converting said acetate functional groups on said surface to hydroxyl functional groups; and    (c) contacting said surface displaying hydroxyl functional groups with at least two different ligands to produce said array;    with the proviso that said method further comprises converting said hydroxyl functional groups to ligand reactive functional groups when said hydroxyl functional groups do not covalently bond to said ligands upon contact;    wherein the functionalized substrate surface has a high contact angle.    
     
     
         37 . The method according to  claim 36 , wherein the contact angle is at least about 45°.  
     
     
         38 . The method according to  claim 36 , wherein the contact angle is at least about 50°.  
     
     
         39 . The method according to  claim 36 , wherein the contact angle is at least about 60°.  
     
     
         40 . The method according to  claim 36 , wherein the contact angle is at least about 65°.  
     
     
         41 . The method according to  claim 36 , wherein the contact angle is at least about 70°.  
     
     
         42 . The method according to  claim 36 , wherein the contact angle is at least about 75°.  
     
     
         43 . The method according to  claim 36 , wherein the contact angle is at least about 80°.  
     
     
         44 . The method according to  claim 36 , wherein the contact angle is at least about 90°.  
     
     
         45 . The method according to  claim 36 , wherein the contact angle is at least about 99°.