US2007059836A1PendingUtilityA1

Compositions and methods for regulating genes of interest

61
Assignee: BLACK CHARLES A JRPriority: Jun 25, 1997Filed: Nov 9, 2006Published: Mar 15, 2007
Est. expiryJun 25, 2017(expired)· nominal 20-yr term from priority
Inventors:Charles Black
C12N 15/67A61P 35/00A61P 43/00C12N 15/113C12N 15/63
61
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Claims

Abstract

Compositions and methods for regulating genes of interest are provided. The compositions comprise sense-antisense double stranded nucleic acid molecules, which have homology to a target nucleic acid sequence in a cell. The compositions and methods are useful for the treatment of disease and for preventing the proliferation of neoplastic cells.

Claims

exact text as granted — not AI-modified
1 - 12 . (canceled)  
     
     
         13 . A method comprising introducing a double stranded nucleic acid molecule into a cell, wherein said double stranded nucleic acid molecule comprises a first strand comprising an RNA sequence and a second strand comprising a polynucleotide bound to said RNA sequence, further wherein said polynucleotide shares up to 100% homology to said first strand and to a target molecule.  
     
     
         14 . The method of  claim 13 , wherein said polynucleotide is RNA or DNA.  
     
     
         15 . The method of  claim 14 , wherein said polynucleotide is DNA.  
     
     
         16 . The method of  claim 14 , wherein said polynucleotide is RNA.  
     
     
         17 . The method of  claim 14 , wherein said polynucleotide comprises modified phosphates.  
     
     
         18 . The method of  claim 14 , wherein said polynucleotide is a sequence of from 10 to 200 nucleotides.  
     
     
         19 . The method of  claim 14 , wherein said first strand and said second strand comprise mismatched nucleotides.  
     
     
         20 . The method of  claim 14 , wherein said first strand or said second strand comprises an unhybridized overhang region.  
     
     
         21 . The method of  claim 14 , wherein said double stranded nucleic acid molecule comprises a stem-loop structure.  
     
     
         22 . The method of  claim 14 , wherein the target molecule is an RNA selected from the group consisting of a viral RNA, an mRNA, and a non-coding RNA.  
     
     
         23 . A cell produced by the process of introducing a double stranded nucleic acid molecule into a target cell, wherein said double stranded nucleic acid molecule comprises a first strand comprises an RNA sequence and a second strand comprises a polynucleotide bound to said RNA sequence, further wherein said polynucleotide shares up to 100% homology to said first strand and to a target molecule.  
     
     
         24 . The method of  claim 23 , wherein said polynucleotide is RNA or DNA.  
     
     
         25 . The method of  claim 24 , wherein said polynucleotide is DNA.  
     
     
         26 . The method of  claim 24 , wherein said polynucleotide is RNA.  
     
     
         27 . The method of  claim 24 , wherein said polynucleotide comprises modified phosphates.  
     
     
         28 . The cell of  claim 24 , wherein polynucleotide an antisense strand from 10 to 200 nucleotides.  
     
     
         29 . The cell of  claim 24 , wherein said first strand and said second strand comprise mismatched nucleotides.  
     
     
         30 . The cell of  claim 24 , wherein said first strand or said second strand comprises an unhybridized overhang region.  
     
     
         31 . The cell of  claim 24 , wherein said double stranded nucleic acid molecule comprises a stem-loop structure.  
     
     
         32 . The cell of  claim 24 , wherein said target cell is selected from the group consisting of an animal cell or a plant cell.

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