Human salivary gland-origin stem cell

Abstract

A novel human stem cell which can be differentiated to cells constituting a plurality of human organs including human liver is disclosed. The human stem cell according to the present invention is originated from human salivary gland, which is CD49f-positive, and which can be differentiated to (1) a nestin-positive and albumin-positive cell, (2) an insulin-positive cell and (3) a glucagon-positive cell by culture in vitro.

1 . An isolated human stem cell originated from human salivary gland, which is CD49f-positive, and which can be differentiated to (1) a nestin-positive and albumin-positive cell, (2) an insulin-positive cell and (3) a glucagon-positive cell by culture in vitro.  
     
     
         2 . A human stem cell which is nestin-positive and albumin-positive, which is induced by culturing a human salivary gland cell in vitro, and which can be differentiated to an insulin-positive cell and a glucagon-positive cell by culture in vitro.  
     
     
         3 . An insulin-positive cell induced by culturing said human stem cell according to  claim 2  in vitro.  
     
     
         4 . A glucagon-positive cell induced by culturing said human stem cell according to  claim 2  in vitro.  
     
     
         5 . The cell according to any one of  claims 1  to  4 , which is originated from an adult.  
     
     
         6 . A method for obtaining human stem cells, comprising collecting CD49f-positive cells from cells originated from human salivary gland tissue, and culturing the cells in the presence of epidermal growth factor to induce human stem cells originated from human salivary gland, which are CD49f-positive, and which can be differentiated to (1) nestin-positive and albumin-positive cells, (2) insulin-positive cells and (3) glucagon-positive cells by culture in vitro, and collecting the induced human stem cells.  
     
     
         7 . A method for obtaining human stem cells, comprising culturing in vitro, in the presence of fibroblast growth factor, epidermal growth factor and leukemia inhibitory factor, human stem cells originated from human salivary gland, which are CD49f-positive, and which can be differentiated to (1) nestin-positive and albumin-positive cells, (2) insulin-positive cells and (3) glucagon-positive cells by culture in vitro, to induce nestin-positive and albumin-positive cells, and collecting the induced nestin-positive and albumin-positive cells.  
     
     
         8 . A method for obtaining insulin-positive cells, comprising culturing in vitro human stem cells which are nestin-positive and albumin-positive, which are induced by culturing human salivary gland cells in vitro, and which can be differentiated to insulin-positive cells and glucagon-positive cells by culture in vitro to induce insulin-positive cells, and collecting the induced insulin-positive cells.  
     
     
         9 . A method for obtaining glucagon-positive cells, comprising culturing in vitro human stem cells which are nestin-positive and albumin-positive, which are induced by culturing human salivary gland cells in vitro, and which can be differentiated to insulin-positive cells and glucagon-positive cells by culture in vitro to induce glucagon-positive cells, and collecting the induced glucagon-positive cells.  
     
     
         10 . The method according to  claim 8  or  9 , wherein said culturing in vitro is carried out by spheroid culture.