Nucleotide sequences which encode the pfk gene

Abstract

The present invention is directed to nucleotide sequences coding for phosphofructokinase which have been Corynebacterium glutamicum , and a process for the production of an L-amino acid comprising culturing a coryneform bacteria comprising an overexpressed pfk gene, wherein said pfk gene comprises a polynucleotide having a nucleotide sequence which is at least 90% identical to the nucleotide of SEQ ID NO: 1 encoding a polypeptide having the enzymatic activity of a phosphofructokinase, accumulating said L-amino acid in the medium or in the cells of said bacterium, and isolated said L-amino acid.

1 - 20 . (canceled)  
     
     
         21 . A process for the production of an L-amino acid comprising the following steps: 
 a) culturing a coryneform bacterium comprising an overexpressed pfk gene, wherein said pfk gene comprises a polynucleotide having a nucleotide sequence which is at least 90% identical to the nucleotide sequence of SEQ ID NO: 1 encoding a polypeptide having the enzymatic activity of a phosphofructokinase, and comprising one or more overexpressed genes selected from the group consisting of the dapA gene of  Corynebacterium glutamicum  encoding dihydrodipicolinate synthase, the pyc gene of  Corynebacterium glutamicum  encoding pyruvate carboxylase, the tpi gene of  Corynebacterium glutamicum  encoding triose phosphate isomerase, the gap gene of  Corynebacterium glutamicum  encoding glyceraldehyde 3-phosphate dehydrogenase and the pgk gene of  Corynebacterium glutamicum  encoding 3-phosphoglycerate kinase,    b) accumulating said L-amino acid in the medium or in the cells of said bacterium    c) isolating said L-amino acid.    
     
     
         22 . The process of claim  1 , wherein said polypeptide having the enzymatic activity of a phosphofructokinase comprises the amino acid sequence of SEQ ID NO: 2.  
     
     
         23 . The process of claim  1 , wherein said polynucleotide comprises the nucleotide sequence of nucleotides 68 to 1057 of SEQ ID NO: 1.  
     
     
         24 . The process of claim  1 , wherein said polynucleotide comprises the nucleotide of SEQ ID NO: 1.  
     
     
         25 . The process of claim  1 , wherein said overexpression is achieved by increasing the copy number of said pfk gene or by operably linking said pfk gene to a promoter.