Method and device for preparing a biological sample for biological analyses

Abstract

The method and devices are for preparing a biological sample for biological analyses. The biological sample has at least one amino acid sequence from an organism. The amino acid sequence is a subject of degradation. The biological sample is physically prepared to enable altering a secondary structure of the amino acid sequence to a deactivated secondary structure, and altering the secondary structure of the amino acid sequence to a deactivated secondary structure by heating the biological sample.

1 . A method for preparing a biological sample for biological analyses, the biological sample having an one amino acid sequence from an organism, the amino acid sequence being a subject of degradation, the method comprising: 
 physically preparing the biological sample to enable altering a secondary structure of the amino acid sequence to a deactivated secondary structure, and    altering the secondary structure of the amino acid sequence to a deactivated secondary structure by heating the biological sample.    
     
     
         2 . The method according to  claim 1 , wherein the method further comprises step of physically preparing comprises shaping the biological sample to enable altering a secondary structure of the amino acid sequence to a deactivated secondary structure.  
     
     
         3 . The method according to  claim 2 , wherein the method further comprises step of shaping the biological sample comprises creating a volume comprising the biological sample.  
     
     
         4 . The method according to  claim 3 , wherein the method further comprises step of creating the volume by filling the volume with a filler presenting a similar dielectric constant as the biological sample.  
     
     
         5 . The method according to  claim 4 , wherein the method further comprises step of the heating is microwave heating.  
     
     
         6 . The method according to  claim 3 , wherein the method further comprises step of creating the volume by designing the shape to present a large heat transfer surface in relation to the volume of the shape.  
     
     
         7 . The method according to  claim 6 , wherein the method further comprises step of creating the volume by shaping the biological sample to a slice.  
     
     
         8 . The method according to  claim 6 , wherein the method further comprises step of the creating the volume by providing a thin and oblong shape to the biological sample.  
     
     
         9 . The method according to  claim 2 , wherein the method further comprises step of heating by contact heat conduction.  
     
     
         10 . The method according to  claim 9 , wherein the contact heat conduction is done by at least of one gas, condensing gas, a fluid and at least one warm plate transferring heat into the biological sample.  
     
     
         11 . The method according to  claim 2 , wherein the method further comprises step of heating by using radiation.  
     
     
         12 . The method according to  claim 7 , wherein the method further comprises step of creating the volume by cutting the biological sample.  
     
     
         13 . The method according to  claim 7 , wherein the method further comprises step of creating the volume by pressing the biological sample.  
     
     
         14 . The method according to  claim 2 , wherein prior to creating the volume, freezing the biological sample.  
     
     
         15 . The method according to  claim 14 , wherein after freezing the biological sample, warming the biological sample to a sub zero temperature allowing cutting the sample.  
     
     
         16 . The method according to  claim 15 , wherein after warming the biological sample, cutting the biological sample.  
     
     
         17 . The method according to  claim 6 , wherein the method further comprises step of creating the volume by placing the biological sample in a container, vacuuming the container, and sealing the container.  
     
     
         18 . The method according to  claim 6 , wherein the method further comprises step of creating the volume by leading the biological sample into a tube, presenting heating means for heat transfer into the biological sample.  
     
     
         19 . The method according to  claim 2 , wherein the heating exceeds a temperature similar to the temperature at which the secondary structure of the amino acid sequence is altered to the deactivated secondary structure.  
     
     
         20 . A device for preparing a biological sample for biological analyses, the biological sample has at least one amino acid sequence from an organism, the one amino acid sequence is being a subject of degradation, comprising: 
 a power transfer location for receiving the biological sample, and    heating means for altering the secondary structure of the amino acid sequence to a deactivated secondary structure, the heating means being responsive for heating the power transfer location.    
     
     
         21 . Device according to  claim 20 , wherein the biological sample has a container.  
     
     
         22 . The device according to  claim 20 , wherein the power-transfer location is constituted by a chamber.  
     
     
         23 . The device according to  claim 22 , wherein the chamber presents one of a circular cross section, a rectangular cross section, and a triangular cross section.  
     
     
         24 . The device according to  claim 22 , wherein the device has an opening defined therein.  
     
     
         25 . The device according to  claim 24 , wherein the device has a second opening defined therein for allowing the biological sample to exit the chamber.  
     
     
         26 . The device according to  claim 21 , wherein an inner wall of the chamber is essentially in contact with the container, when the container is inserted into the chamber.  
     
     
         27 . The device according to  claim 21 , wherein the container is one of a needle and a tube.  
     
     
         28 . The device according to  claim 24 , wherein the opening presents a sealing, allowing insertion of the container into the chamber.  
     
     
         29 . The device according to  claim 22 , wherein the chamber has a plurality of openable matching parts and a locking means for maintaining the plurality of openable matching parts in a locked state.  
     
     
         30 . The device according to  claim 22 , wherein the device has a steam generator that has an inlet defined therein and the chamber has a steam inlet defined therein.  
     
     
         31 . The device according to  claim 20 , wherein the power-transfer location is constituted by at least one plate.  
     
     
         32 . The device according to  claim 20 , wherein the heating means is one of a radiation generator and a micro wave generator.  
     
     
         33 . The device according to  claim 20 , wherein the power-transfer location presents a large power transfer surface.