Dipeptide production method, L-amino acid amide hydrolase used therein, and production method of L-amino acid amide hydrolase

Abstract

A process for industrially advantageously producing a dipeptide via a convenient pathway starting with less expensive and easily available materials is provided. A dipeptide is produced from an L-amino acid amide and an L-amino acid by using a culture of a microbe capable of synthesizing the dipeptide from the L-amino acid amide and the L-amino acid, microbial cells separated from the culture or a treated microbial cell product from the microbe. An L-amino acid amide hydrolase is obtained from a microbe belonging to the genus erwinia, genus Rhodococcus, genus Chryseobacterium, genus Micrococcus, genus Cryptococcus, genus Trichosporion, genus Rhodosporidium, genus Sporobolomyces, genus Tremela, genus Torulaspora, genus Sterigmatomyces or genus Rhodotorula. The hydrolase catalyzes a reaction that produces a dipeptide from an L-amino acid amide and an L-amino acid.

1 - 8 . (canceled)  
     
     
         9 . An L-amino acid amide hydrolase obtained from a microbe belonging to the genus  Erwinia,  genus  Rhodococcus,  genus  Chryseobacterium,  genus  Micrococcus,  genus  Cryptococcus,  genus  Trichosporon,  genus  Rhodosporidium,  genus  Sporobolomyces,  genus  Tremela,  genus  Torulaspora,  genus  Sterigmatomyces  or genus  Rhodotorula,  which catalyzes a reaction that produces a dipeptide from an L-amino acid amide and an L-amino acid.  
     
     
         10 . A production method of L-amino acid amide hydrolase, comprising: culturing a microbe belonging to the genus  Erwinia,  genus  Rhodococcus,  genus  Chryseobacterium,  genus  Micrococcus,  genus  Cryptococcus,  genus  Trichosporon,  genus  Rhodosporidium,  genus  Sporobolomyces,  genus  Tremela,  genus  Torulaspora,  genus  Sterigmatomyces  or genus  Rhodotorula  in a medium, and accumulating in the medium and/or cells an L-amino acid amide hydrolase that catalyzes a reaction that produces a dipeptide from an L-amino acid amide and an L-amino acid.  
     
     
         11 . (canceled)