Method for producing a population of homozygous stem cells having a pre-selected immunotype and/or genotype, cells suitable for transplant derived therefrom, and materials and methods using same

Abstract

A method of producing a homogenous population of homozygous stem (HS) cells pre-selected for immunotype and/or genotype from donor cells is described herein. The invention relates to methods of using immunohistocompatible HS cells for diagnosis, therapeutic and cosmetic transplantation, and the treatment of various genetic diseases, neurodegenerative diseases, traumatic injuries and cancer. The invention further relates to methods for using histocompatible HS stem cells pre-selected for a non-disease genotype for prophylactic and therapeutic intervention including, but not limited to, therapeutic and cosmetic transplantation, and the treatment of various genetic diseases, neurodegenerative diseases, and cancer. Furthermore, the invention relates to a catalogued transplant depository of HS cells derived from multiple donors, each of the HS cells being homozygous for a unique HLA haplotype, for the purpose of having a constant, reliable, comprehensive supply of immunohistocompatible cells for diagnosis, treatment and/or transplantation.

1 - 28 . (canceled)  
     
     
         29 . A method for producing a homozygous stem (HS) cell depository, wherein each HS cell that produces a cell line has alleles that are homozygous for a Major Histocompatibility Complex (MHC) haplotype, comprising: 
 (a) selecting donors;    (b) determining the MHC haplotype of each donor;    (c) mitotically activating non-fertilized female post-meiosis I diploid germ cells obtained from each donor to develop multiple blastocyst-like masses, each of which contains an inner cell mass (ICM) that is homozygous for a particular MHC haplotype, wherein the non-fertilized female post-meiosis I diploid germ cells are activated by calcium ionophore and sham intracytoplasmic sperm injection (ICSI);    (d) isolating HS cells from the ICM obtained from each donor;    (e) culturing the isolated HS cells to obtain HS cell lines;    (f) determining the MHC haplotype of each HS cell line; and    (g) cataloging the MHC haplotype of each HS cell line obtained in (e).    
     
     
         30 . (canceled)  
     
     
         31 . The method of  claim 29 , wherein the donors are mammalian.  
     
     
         32 . The method of  claim 31 , wherein the donors are human.  
     
     
         33 . The method of  claim 31 , wherein the donors are non-human.