Human Oncogene Induced Secreted Protein I

Abstract

The present invention relates to a novel protein, the Human Oncogene Induced Secreted Protein I (“HOIPS I”) protein. In particular, isolated nucleic acid molecules are provided encoding the human HOIPS I protein. HOIPS I polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. Antibodies to human HOIPS I are also provided, as are diagnostic methods for detecting abnormal cell proliferation and differentiation disorders and therapeutic methods for treating the same.

1 . An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of: 
 (a) a nucleotide sequence encoding a polypeptide comprising amino acids from about −20 to about 142 in SEQ ID NO:2;    (b) a nucleotide sequence encoding a polypeptide comprising amino acids from about −19 to about 142 in  FIG. 1  SEQ ID NO:2;    (c) a nucleotide sequence encoding a polypeptide comprising amino acids from about 1 to about 142 in SEQ ID NO:2;    (d) a nucleotide sequence encoding a polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97825;    (e) a nucleotide sequence encoding the mature HOIPS I polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97825; and    (f) a nucleotide sequence complementary to any of the nucleotide sequences in (a), (b), (c), (d) or (e).    
     
     
         2 . The nucleic acid molecule of  claim 1 , wherein said polynucleotide has the nucleotide sequence in SEQ ID NO:1.  
     
     
         3 . An isolated nucleic acid molecule comprising a polynucleotide which hybridizes under stringent hybridization conditions to a polynucleotide having a nucleotide sequence identical to a nucleotide sequence in (a), (b), (c), (d), (e) or (f) of  claim 1  wherein said polynucleotide which hybridizes does not hybridize under stringent hybridization conditions to a polynucleotide having a nucleotide sequence consisting of only A residues or of only T residues.  
     
     
         4 . An isolated nucleic acid molecule comprising a polynucleotide which encodes the amino acid sequence of an epitope-bearing portion of a HOIPS I polypeptide having an amino acid sequence in (a), (b), (c), (d), or (e) of  claim 1 .  
     
     
         5 . A method for making a recombinant vector comprising inserting an isolated nucleic acid molecule of  claim 1  into a vector.  
     
     
         6 . A recombinant vector produced by the method of  claim 5 .  
     
     
         7 . A method of making a recombinant host cell comprising introducing the recombinant vector of  claim 6  into a host cell.  
     
     
         8 . A recombinant host cell produced by the method of  claim 7 .  
     
     
         9 . A recombinant method for producing a HOIPS I polypeptide, comprising culturing the recombinant host cell of  claim 8  under conditions such that said polypeptide is expressed and recovering said polypeptide.  
     
     
         10 . An isolated HOIPS I polypeptide having an amino acid sequence at least 95% identical to a sequence selected from the group consisting of: 
 (a) amino acids from about −20 to about 142 in SEQ ID NO:2;    (b) amino acids from about −19 to about 142 in SEQ ID NO:2;    (c) amino acids from about 1 to about 142 in SEQ ID NO:2;    (d) the amino acid sequence of the HOIPS I polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97825;    (e) the amino acid sequence of the mature HOIPS I polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97825; and    (f) the amino acid sequence of an epitope-bearing portion of any one of the polypeptides of (a), (b), (c), (d), or (e).    
     
     
         11 . The isolated polypeptide of  claim 10 , which is produced or contained in a recombinant host cell.  
     
     
         12 . The isolated polypeptide of  claim 11 , wherein said recombinant host cell is mammalian.  
     
     
         13 . An isolated antibody that binds specifically to the polypeptide of  claim 10 .  
     
     
         14 . A pharmaceutical composition comprising: 
 (a) the antibody of  claim 13 , and    (b) a pharmaceutically acceptable carrier.    
     
     
         15 . A method of diagnosing a cell proliferative or differentiation disorder comprising using the polynucleotide of  claim 3  to determine the level of HOIPS I mRNA in a biological sample, wherein an increase in the level of HOIPS I mRNA compared to the standard is indicative of a cell proliferative or differentiation disorder.  
     
     
         16 . The method of  claim 15  wherein the cell proliferative or differentiation disorder is cancer.  
     
     
         17 . A method of treating a subject having or developing a HOIPS I related cell proliferative and/or differentiation disorder comprising administering the antibody of  claim 13 .  
     
     
         18 . A method of treating a subject having or developing a HOIPS I related cell proliferative or differentiation disorder comprising administering the recombinant vector of  claim 6  to an abnormally proliferating cell or cells.