Compositions for regenerating defective or absent myocardium

Abstract

Compositions of the invention for regenerating defective or absent myocardium comprise an emulsified or injectable extracellular matrix composition. The composition can also have an extracellular matrix scaffold component of any formulation, then including also added cells, proteins, or other components to optimize the regenerative process and restore cardiac function. Methods for regenerating defective or absent myocardium apply a composition to a site of myocardium in need of regeneration using a delivery mode appropriate for the particular formulation.

1 . A method of making an emulsified or injectable extracellular matrix composition from a mammalian or synthetic source comprising emulsifying a mammalian or synthetic extracellular matrix scaffold component from a mammalian or synthetic source and a first additional component selected from the group consisting of a collagen present in myocardial tissue, a collagen type IV protein, a collagen type VIII protein, a collagen type X protein, a multiplexin, a matrix heparin sulfate protoeglycan, a laminin, a nidogen, an agin, and a perlecan, and storing the composition as an emulsion for administration to a patient.  
     
     
         2 . The method as in  claim 1 , further comprising adding a second additional component to the composition before, after or during emulsification.  
     
     
         3 . The method as in  claim 2 , wherein the second additional component is selected from the group consisting of 
 a) a cell,    b) a peptide, polypeptide, or protein,    c) a vector having a DNA capable of targeted expression of a selected gene, and    d) a nutrient, a sugar, a fat, a lipid, an amino acid, a nucleic acid, a ribo-nucleic acid, an organic molecule, an inorganic molecule, a small molecule, a drug, or a bioactive molecule.    
     
     
         4 . The method as in  claim 1  wherein the emulsion comprises mammalian extracellular matrix selected from the group consisting of small intestine submucosa (SIS), urinary bladder submucosa (UBS), urinary basement membrane (UBM), liver basement membrane (LBM), stomach submucosa (SS), collagen from animal sources, collagen from plant sources, synthesized extracellular matrix in cultures from cells (Matrigel™), dermal extracellular matrix material, subcutaneous extracellular matrix material, large intestine extracellular matrix material, placental extracellular matrix material, ornamentum extracellular matrix material, heart extracellular matrix material, and lung extracellular matrix material.  
     
     
         5 . The method as in  claim 1 , wherein the emulsion comprises synthetic extracellular matrix selected from the group consisting of polyethylene terephthalate fiber (Dacron), polytetrafluoroethylene (PTFE), glutaraldehyde-cross linked pericardium, polylactate (PLA), polyglycol (PGA), hyaluronic acid, polyethylene glycol (PEG), polyethelene, a nucleic acid polymer, nitinol, and collagen from non-animal sources.