Compositions for regenerating defective or absent myocardium

Abstract

Compositions of the invention for regenerating defective or absent myocardium comprise an emulsified or injectable extracellular matrix composition. The composition can also have an extracellular matrix scaffold component of any formulation, then including also added cells, proteins, or other components to optimize the regenerative process and restore cardiac function. Methods for regenerating defective or absent myocardium apply a composition to a site of myocardium in need of regeneration using a delivery mode appropriate for the particular formulation.

1 . A method of making a composition comprising at least a portion of an extracellular matrix scaffold component from a mammalian source and a first additional component selected from the group consisting of a collagen Present in myocardial tissue, a collagen type IV protein, a collagen type VIII protein, a collagen type X protein, a multiplexin, a matrix heparin sulfate protoeglycan, a laminin, a nidogen, an agin, and a perlecan, and a second additional component selected from the groups consisting of: 
 a) a plurality of synthetic extracellular matrix-like scaffold-forming molecules    b) a cell,    c) a peptide, polypeptide, or protein, and    d) a vector having a DNA capable of targeted expression of a selected gene, and    e) a nutrient, a sugar, a fat, a lipid, an amino acid, a nucleic acid, a ribo-nucleic acid, an organic molecule, an inorganic molecule, a small molecule, a drug, or a bioactive molecule,    said method comprising contacting the extracellular matrix scaffold component with at least one first additional component and at least one second additional component.    
     
     
         2 . The method of  claim 1 , wherein said extracellular matrix scaffold component is formulated in a material form selected from the group consisting of a solid sheet, multilaminate sheets, a gel, an emulsion, an injectable solution, a fluid, a paste, a powder, a plug, a strand, a suture, a coil, a cylinder, a weave, a strip, a spray, a vapor, a patch, a sponge, a cream, a coating, a lyophilized material, and a vacuum-pressed material.  
     
     
         3 . The method of  claim 1 , wherein said first or second additional component is formulated in a material form selected from the group consisting of a gel, an emulsion, an injectable solution, a fluid, a paste, a powder, a plug, a strand, a spray, a vapor, a cream, a coating, a lyophilized material and a vacuum-pressed material.  
     
     
         4 . The method of  claim 1 , wherein the extracellular matrix scaffold component is from a source selected from the group consisting of small intestine submucosa (SIS), urinary bladder submucosa (UBS), urinary basement membrane (UBM), liver basement membrane (LBM), stomach submucosa (SS), collagen from animal sources, collagen from plant sources, synthesized extracellular matrix in cultures from cells (Matrigel™), dermal extracellular matrix material, subcutaneous extracellular matrix material, large intestine extracellular matrix material, placental extracellular matrix material, ornamentum extracellular matrix material, heart extracellular matrix material, and lung extracellular matrix material.  
     
     
         5 . The method of  claim 1 , wherein the second additional component is a plurality of synthetic extracellular matrix-like scaffold-forming molecules, and said synthetic molecules are selected from the group consisting of polyethylene terephthalate fiber (Dacron), polytetrafluoroethylene (PTFE), glutaraldehyde-cross linked pericardium, polylactate (PLA), polyglycol (PGA), hyaluronic acid, polyethylene glycol (PEG), polyethelene, a nucleic acid polymer, nitinol, and collagen from non-animal sources.