US2007011760A1PendingUtilityA1

Nucleic acids that control seed and fruit development in plants

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Assignee: UNIV CALIFORNIAPriority: May 1, 1998Filed: Oct 20, 2005Published: Jan 11, 2007
Est. expiryMay 1, 2018(expired)· nominal 20-yr term from priority
Inventors:Robert L. FischerNir OhadTomohiro KiyosueRamin YadegariLinda MargossianJohn HaradaRobert B. Goldberg
C12N 15/8267C07K 14/415C07K 2319/00C12N 15/8261C12N 15/8287Y02A40/146
54
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Claims

Abstract

The invention provides methods of controlling endosperm development in plants.

Claims

exact text as granted — not AI-modified
1 . An isolated nucleic acid molecule comprising a FIE polynucleotide sequence, which polynucleotide sequence specifically hybridizes to SEQ ID NO:1 or SEQ ID NO:3 under stringent conditions.  
     
     
         2 . The isolated nucleic acid molecule of claim I, wherein the FIE polynucleotide is between about at least about 100 nucleotides in length.  
     
     
         3 . The isolated nucleic acid molecule of  claim 1 , wherein the FIE polynucleotide is SEQ ID NO:1.  
     
     
         4 . The isolated nucleic acid molecule of  claim 1 , wherein the FIE polynucleotide is SEQ ID NO:3.  
     
     
         5 . The isolated nucleic acid molecule of  claim 1 , further comprising a plant promoter operably linked to the FIE polynucleotide.  
     
     
         6 . The isolated nucleic acid molecule of  claim 5 , wherein the plant promoter is from a FIE1 gene.  
     
     
         7 . The isolated nucleic acid of  claim 6 , wherein the FIE polynucleotide is linked to the promoter in an antisense orientation.  
     
     
         8 . An isolated nucleic acid molecule comprising a FIE polynucleotide sequence, which polynucleotide sequence encodes FIE polypeptide as shown in SEQ ID NO:2 or SEQ ID NO:4.  
     
     
         9 . a transgenic plant comprising an expression cassette containing a plant promoter operably linked to a heterologous FIE polynucleotide of  claim 1 .  
     
     
         10 . The transgenic plant of  claim 9 , wherein the heterologous FIE polynucleotide encodes a FIE polypeptide.  
     
     
         11 . The transgenic plant of  claim 10 , wherein the FIE polypeptide is as shown in SEQ ID NO:2 or SEQ ID NO:4.  
     
     
         12 . The transgenic plant of  claim 9 , wherein the heterologous FIE polynucleotide is linked to the promoter in an antisense orientation.  
     
     
         13 . The transgenic plant of  claim 9 , wherein the plant promoter is from a FIE gene.  
     
     
         14 . The transgenic plant of  claim 13 , wherein the FIE gene is as shown in SEQ ID NO:1 or SEQ ID NO:3.  
     
     
         15 . A method of modulating endosperm development in a plant, the method comprising introducing into the plant an expression cassette containing a plant promoter operably linked to a heterologous FIE polynucleotide.  
     
     
         16 . The method of  claim 15 , wherein the heterologous FIE polynucleotide encodes an FIE polypeptide.  
     
     
         17 . The method of  claim 16 , wherein the FIE polypeptide has an amino acid sequence as shown in SEQ ID NO:2 or SEQ ID NO:4.  
     
     
         18 . The method of  claim 15 , wherein the heterologous FIE polynucleotide is linked to the promoter in an antisense orientation.  
     
     
         19 . The method of  claim 15 , wherein the heterologous FIE polynucleotide is SEQ ID NO:1 or SEQ ID NO:3.  
     
     
         20 . The method of  claim 15 , wherein the plant promoter is from a FIE gene.  
     
     
         21 . The method of  claim 15 , wherein the expression cassette is introduced into the plant through a sexual cross.

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