Complementary DNAs

Abstract

The sequences of extended cDNAs encoding secreted proteins are disclosed. The extended cDNAs can be used to express secreted proteins or portions thereof or to obtain antibodies capable of specifically binding to the secreted proteins. The extended cDNAs may also be used in diagnostic, forensic, gene therapy, and chromosome mapping procedures. The extended cDNAs may also be used to design expression vectors and secretion vectors.

1 . An isolated or purified polypeptide: 
 a) comprising at least 50, 75, 100 or 150 consecutive amino acids of SEQ ID NO: 104;    b) comprising amino acids 1 to 51 of SEQ ID NO: 104;    c) consisting of amino acids 1 to 51 of SEQ ID NO: 104;    d) comprising SEQ ID NO: 104;    e) consisting of SEQ ID NO: 104;    f) comprising the amino acid sequence encoded by the insert from deposited clone 108-006-5-0-G2-FL in ATCC accession number 98921; or    g) comprising an amino acid sequence has at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% identity to the polypeptide of SEQ ID NO:104.    
     
     
         2 . The polypeptide according to  claim 1 , wherein said polypeptide comprises amino acids 1 to 51 of SEQ ID NO:104.  
     
     
         3 . The polypeptide according to  claim 1 , wherein said polypeptide consists of amino acids 1 to 51 of SEQ ID NO:104.  
     
     
         4 . The polypeptide according to  claim 1 , wherein said polypeptide comprises the sequence of SEQ ID NO:104.  
     
     
         5 . The polypeptide according to  claim 4 , wherein said polypeptide consists of an amino acid sequence of SEQ ID NO:104.  
     
     
         6 . The polypeptide according to  claim 1 , wherein said polypeptide comprises an amino acid sequence encoded by the insert from deposited clone 108-006-5-0-G2-FL in ATCC accession number 98921.  
     
     
         7 . The polypeptide of  claim 1 , wherein said polypeptide has at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% identity to the polypeptide of SEQ ID NO:104.  
     
     
         8 . The polypeptide of  claim 1 , wherein said polypeptide is a neuronal protein involved in cellular proliferation and differentiation.  
     
     
         9 . A method of making a polypeptide comprising the steps of: 
 a) obtaining a cDNA encoding a polypeptide: 
 i) comprising at least 50, 75, 100 or 150 consecutive amino acids of SEQ ID NO: 104;  
 ii) comprising amino acids 1 to 51 of SEQ ID NO: 104;  
 iii) consisting of amino acids 1 to 51 of SEQ ID NO: 104;  
 iv) comprising SEQ ID NO. 104;  
 v) consisting of SEQ ID NO: 104;  
 vi) comprising the amino acid sequence encoded by the insert from deposited clone 108-006-5-O-G2-FL in ATCC accession number 98921; or  
 vii). comprising an amino acid sequence has at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% identity to the polypeptide of SEQ ID NO:104;  
   b) inserting said cDNA in an expression vector such that said cDNA is operably linked to a promoter; and    c) introducing said expression vector into a host cell whereby said host cell produces the protein encoded by said cDNA.    
     
     
         10 . The method of  claim 9 , further comprising the step of isolating said polypeptide.