US2006286598A1PendingUtilityA1

Isolated CEL II endonuclease

Individually held — no corporate assignee on recordPriority: Oct 18, 2002Filed: Aug 22, 2006Published: Dec 21, 2006
Est. expiryOct 18, 2022(expired)· nominal 20-yr term from priority
C12Q 1/6827C12N 9/22
56
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Claims

Abstract

The present invention relates to the isolation and characterization of CEL I and CEL II endonuclease proteins. Methods and kits for identifying mismatches in double-stranded DNA are also provided.

Claims

exact text as granted — not AI-modified
1 . A method for detecting the presence of mismatches in double-stranded DNA comprising contacting a sample containing double-stranded DNA with an isolated CEL II endonuclease, separating the product of the CEL II endonuclease digestion, and detecting said product wherein an increase in the number of DNA fragments generated in the presence of the CEL II endonuclease is indicative of a mismatch in said DNA.  
     
     
         2 . A kit for detecting the presence of mismatches in double-stranded DNA comprising an isolated CEL II endonuclease enzyme.

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