Alternative splicing factors polynucleotides, polypeptides and uses thereof

Abstract

The invention provides isolated polynucleotides and their encoded proteins that are involved in splicing or modulating splicing activity. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering splicing protein content and/or composition of plants.

1 . An isolated nucleic acid encoding a polypeptide having RNA splicing activity comprising: 
 (a) a polynucleotide that encodes a polypeptide of SEQ ID NOS: 2 or 4;    (b) a polynucleotide that encodes a polypeptide having at least 90% identity to SEQ ID NOS: 2 or 4;    (c) a polynucleotide comprising the sequence set forth in SEQ ID NO: 30.    
     
     
         2 . A vector comprising at least one nucleic acid of  claim 1 .  
     
     
         3 . A recombinant expression cassette comprising a nucleic acid of  claim 1  operably linked to a promoter.  
     
     
         4 . A host cell comprising the recombinant expression cassette of  claim 3 , wherein said host cell is a plant cell or a bacterial cell.  
     
     
         5 . The host cell of  claim 4  wherein said host cell is a plant cell.  
     
     
         6 . The plant cell of  claim 5  wherein said plant cell is a corn, soybean, sorghum, sunflower, safflower, wheat, rice, alfalfa, or  Brassica  cell.  
     
     
         7 . A transgenic plant comprising at least one expression cassette of  claim 3 .  
     
     
         8 . A transgenic seed from the plant of  claim 7 .  
     
     
         9 . A transgenic plant produced by growing the seed of  claim 8 .  
     
     
         10 . A method of increasing the splicing of a Geminivirus comprising introducing the isolated nucleic acid of  claim 1  into a plant cell.  
     
     
         11 . The method of  claim 10  wherein the virus is a wheat dwarf or maize streak virus.  
     
     
         12 . The method of  claim 10  wherein Rep A splicing is increased.  
     
     
         13 . An isolated ribonucleic acid sequence encoded by a polynucleotide of  claim 1.