Dendrimer-based DNA extraction methods and biochips

Abstract

The present invention provides a dendrimer-based biochip, wherein a flow channel through which a solution containing biopolymer molecules is flowed is formed in the substrate of the biochip, a plurality of dendrimer molecules one end of each of which is bound to the walls of the flow channel are formed thereon, and probe biopolymer or antibody molecules are bound to the tips of the dendrimer molecules so that, if the probe biopolymer molecules are bound, then target biopolymer molecules can be captured by means of a complementary combination and, if the antibody molecules are bound, then protein can be extracted by means of antigen-antibody reaction, whereby biopolymers can be retrieved in a highly efficient manner.

1 . A dendrimer-based biochip, wherein a flow channel through which a solution containing biopolymer molecules is flowed, is formed in the substrate of said biochip, a plurality of dendrimer molecules, one end of each of which is bound to the walls of said flow channel, are formed thereon, probe biopolymer or antibody molecules are bound to the tips of said dendrimer molecules and, if said probe biopolymer molecules are bound, then target biopolymer molecules are captured by means of a complementary combination and, if said antibody molecules are bound, then protein is extracted by means of antigen-antibody reaction.  
   
   
       2 . The dendrimer-based biochip of  claim 1 , wherein said dendrimer is a multibranched polyamide amine dendrimer produced by providing aminosilane treatment on the walls of said flow channel and overlaying a film of amidoamine, which is produced by the reaction of methyl acrylate with ethylenediamine, upon the aminosilane-treated area as a dendron unit.  
   
   
       3 . The dendrimer-based biochip of  claim 1 , wherein the generation of said dendrimer molecules is second or higher.  
   
   
       4 . The dendrimer-based biochip of  claim 2 , wherein the generation of said dendrimer molecules is second or higher.  
   
   
       5 . The dendrimer-based biochip of  claim 1 , wherein concavities and convexities are formed on the walls of said flow channel.  
   
   
       6 . The dendrimer-based biochip of  claim 2 , wherein concavities and convexities are formed on the walls of said flow channel.  
   
   
       7 . The dendrimer-based biochip of  claim 3 , wherein concavities and convexities are formed on the walls of said flow channel.