Modulators of the P2Y10 receptor useful in altering T lymphocyte function

Abstract

The present invention relates to modulators of P2Y10. Various modulators are disclosed, including an antibody that binds specifically to P2Y10. The present invention also relates to methods of activating resting T lymphocytes and inhibiting the proliferation of activated T lymphocytes. Methods of upregulating trascription of P2Y10 mRNA in a resting T lymphocyte, methods of inducing expression of P2Y10 on the surface of a resting T lymphocyte and methods of activating a T lymphocyte are disclosed. Also disclosed are pharmaceuticals and methods of treating an immune disease.

1 .- 5 . (canceled)  
     
     
         6 . A method of identifying a modulator of a P2Y10 polypeptide comprising 
 (a) measuring the ability of a test molecule to bind to a P2Y10 polypeptide; and    (b) measuring one or more of: 
 (i) the ability of said test molecule to activate a resting T lymphocyte;  
 (ii) the ability of said test molecule to induce surface expression of P2Y10 on a T lymphocyte;  
 (iii) the ability of said test molecule to stimulate P2Y10 mRNA expression in a T lymphocyte; and  
 (iv) the ability of said test molecule to inhibit proliferation of an activated T lymphocyte;  
 whereby a test molecule is identified as a modulator if the test molecule binds to a P2Y10 polypeptide and exhibits one or more abilities selected from the group consisting of (i) to (iv).  
   
     
     
         7 . The method of  claim 6 , wherein the modulator associates specifically with residues 171-191 of a P2Y10 polypeptide (SEQ ID NO:3).  
     
     
         8 . The method of  claim 6 , wherein the modulator is an antibody.  
     
     
         9 . The method of  claim 8 , wherein the antibody is produced by hybridoma strain PTA-3975.  
     
     
         10 . (canceled)