US2006228746A1PendingUtilityA1

Composition and method for optimized hybridization using modified solutions

58
Individually held — no corporate assignee on recordPriority: Oct 25, 2001Filed: May 31, 2006Published: Oct 12, 2006
Est. expiryOct 25, 2021(expired)· nominal 20-yr term from priority
Inventors:Theodore R. SanaPaul WolberClotilde Perbost
C12Q 1/6832Y10T436/17
58
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Claims

Abstract

The invention provides a composition, kit and method for hybridizing a probe and target at a temperature lower than their standard hybridization temperature. The chemical component added to the composition has a formula R(NH 2 )C═O, where R is amino or alkyl. A method for use of the chemical component and composition is also disclosed.

Claims

exact text as granted — not AI-modified
1 - 17 . (canceled)  
   
   
       18 . A composition of matter comprising: 
 a) a surface-bound oligonucleotide probe;    b) a polynucleotide target for said probe; and    c) urea.    
   
   
       19 . The composition of matter of  claim 18 , wherein said surface-bound oligonucleotide probe is attached to a surface of a substrate.  
   
   
       20 . The composition of matter of  claim 19 , wherein said substrate is a glass substrate.  
   
   
       21 . The composition of matter of  claim 19 , wherein said substrate comprises an array of at least 1000 surface-bound oligonucleotide probes.  
   
   
       22 . The composition of matter of  claim 18 , wherein said surface-bound oligonucleotide probe is 10 to 100 nucleotides in length.  
   
   
       23 . The composition of matter of  claim 18 , wherein said urea is present in an amount that decreases the standard hybridization temperature of said oligonucleotide probe and said polynucleotide target.  
   
   
       24 . The composition of matter of  claim 18 , wherein said hybridization reagent is at a temperature in the range of 35° C. to 42° C.  
   
   
       25 . The composition of matter of  claim 18 , wherein said urea is present at a concentration of 2.5 M to 5.5 M.  
   
   
       26 . A kit comprising: 
 an array of surface-bound oligonucleotide probes; and    a hybridization buffer comprising urea.    
   
   
       27 . The kit of  claim 26 , wherein said surface-bound oligonucleotide probes are linked to a glass substrate.  
   
   
       28 . The kit of  claim 26 , wherein said array is an array of at least 1000 surface-bound oligonucleotide probes.  
   
   
       29 . The kit of  claim 26 , wherein said surface-bound oligonucleotide probes are 10 to 100 nucleotides in length.  
   
   
       30 . The kit of  claim 26 , wherein said urea is present at a concentration of 2.5 M to 5.5 M.  
   
   
       31 . A composition of matter comprising: 
 a) a surface-bound oligonucleotide probe;    b) a polynucleotide target for said probe; and    c) acetamide.    
   
   
       32 . The composition of matter of  claim 31 , wherein said surface-bound oligonucleotide probe is attached to a surface of a substrate.  
   
   
       33 . The composition of matter of  claim 32 , wherein said substrate is a glass substrate.  
   
   
       34 . A kit comprising: 
 an array of surface-bound oligonucleotide probes; and    a hybridization buffer comprising acetamide.    
   
   
       35 . The kit of  claim 34 , wherein said surface-bound oligonucleotide probes are linked to a glass substrate.  
   
   
       36 . The kit of  claim 34 , wherein said array is an array of at least 1000 surface-bound oligonucleotide probes.

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