Gram-positive bacteria deprived of HtrA protease activity and their uses

Abstract

The invention concerns bacteria strains, obtained from gram-positive bacteria whereof the genome size is not more than 3.2 Mb, and wherein the HtrA surface protease is inactive. Said strains are useful for expressing exported proteins of interest.

1 - 10 . (canceled)  
     
     
         11 . A method of producing a fermented product, comprising: culturing a bacterial strain with a fermentation substrate under conditions suitable to produce a fermented product, wherein the bacterial strain does not express a functional HtrA.  
     
     
         12 . A method of producing a dietetic food, comprising: culturing a bacterial strain with a substrate under conditions suitable to produce a dietetic food, wherein the bacterial strain does not express a functional HtrA.  
     
     
         13 . A method of producing a medicinal product, comprising: culturing a bacterial strain with a suitable substrate under conditions effective to produce a medicinal product, wherein the bacterial strain does not express a functional HtrA.  
     
     
         14 . A method according to  claim 13 , wherein the medicinal product is a vaccine.