Detection of antibacterial activity in excretory secretory product of adult trichuris suis

Abstract

The present invention provides a heat-stable and protease-resistant antibacterial activity in excretory-secretory products (ESP) of Trichuris suis . The antibacterial activity is not more than 10,000 MW; is resistant to boiling, trypsin, and pronase E; has a bactericidal mode of action; and is effective against Gram positive and Gram negative bacteria, including Escherichia coli, Campylobacter jejuni, Campylobacter coli , and Staphylococcus aureus . The antibacterial activity is useful in applications for killing or inhibiting the growth of microorganisms, in particular bacteria.

1 - 10 . (canceled)  
   
   
       11 . A method for inhibiting a microorganism which comprises: 
 contacting the microorganism with an excretory-secretory product of  Trichuris suis  which comprises at least one antibacterial activity which inhibits the growth of  Campylobacter jejuni  as an assay strain in a growth medium and which is resistant to boiling, freeze-thawing, trypsin, and pronase E in an amount which inhibits the microorganism.    
   
   
       12 . The method of  claim 11  wherein the microorganism is a bacterium.  
   
   
       13 . The method of  claim 11  wherein the antibacterial activity has a minimum inhibitory concentration and a minimum bactericide concentration of the bacterial activity which are about the same.  
   
   
       14 . The method of  claim 11  wherein the antibacterial activity which is not more than 10K MW.  
   
   
       15 . The method of  claim 11  wherein the antibacterial activity inhibits at least 50% of at least one species of cream positive or cream negative bacteria.  
   
   
       16 . The method of  claim 15  wherein the antibacterial activity inhibits a bacteria selected from the group consisting of  Campylobacter jejuni, Campylobacter coli, Escherichia coli , and  Staphylococcus aureus.    
   
   
       17 - 29 . (canceled)