Bioarray chip reaction apparatus and its manufacture

Abstract

This invention provides oligonucleotide based arrays and methods for speciating and phenotyping organisms, for example, using oligonucleotide sequences based on the Mycobacterium tuberculosis rpoB gene. The groups or species to which an organism belongs may be determined by comparing hybridization patterns of target nucleic acid from the organism to hybridization patterns in a database.

1 - 89 . (canceled)  
     
     
         90 . An array comprising test biopolymeric probes for targets of two or more different species, wherein said test biopolymeric probes are immobilized on a surface of a solid support.  
     
     
         91 . The array according to  claim 90 , wherein said test biopolymeric probes are nucleic acid probes.  
     
     
         92 . The array according to  claim 90 , wherein said test biopolymeric probes are peptide probes.  
     
     
         93 . The array according to  claim 90 , wherein said array comprises a single solid support.  
     
     
         94 . The array according to  claim 90 , wherein said test biopolymeric probes are covalently attached to said surface of said substrate.  
     
     
         95 . A method of preparing an array, said method comprising: immobilizing test biopolymeric probes for targets of a first species on a surface of a solid support; and immobilizing test biopolymeric probes for targets of a second species different from said first species on a surface of a solid support; to prepare said array.  
     
     
         96 . The method according to  claim 95 , wherein said immobilizing comprises in situ synthesis of test biopolymeric probes on said surface of a solid support.  
     
     
         97 . A method of detecting the presence of a target in a sample, said method comprising: (a) contacting an array according to  claim 90  having a test biopolymeric probe that specifically binds to said target with a sample suspected of comprising said target under conditions sufficient for binding of said target to said test biopolymeric probe on said array to occur; and (b) detecting the presence of binding complexes on the surface of said array to detect the presence of said target in said sample.  
     
     
         98 . The method according to  claim 97 , wherein said test probe and analyte are nucleic acids.  
     
     
         99 . The method according to  claim 97 , wherein said test probe and analyte are peptides.  
     
     
         100 . A kit for use in an analyte detection assay, said kit comprising: an array according to  claim 90;  and instructions for using said array in an analyte detection assay.  
     
     
         101 . The kit according to  claim 100 , wherein said kit further comprises means for generating targets from two or more different species.  
     
     
         102 . A method comprising reading an array according to  claim 90  that has been contacted by a sample.