Survivin-binding proteins, encoding nucleic acids, and methods of use

Abstract

In accordance with the present invention, there are provided novel Survivin-binding-proteins (SBPs). Nucleic acid sequences encoding such proteins and assays employing same are also disclosed. The invention SBPs can be employed in a variety of ways, for example, for the production of anti-SBP antibodies thereto, in therapeutic compositions and in bioassays methods employing such proteins and/or antibodies. Also provided are transgenic non-human mammals that express the invention protein.

1 . An isolated nucleic acid molecule encoding an SBP1 polypeptide, or functional fragment thereof, selected from the group consisting of: 
 (a) a nucleic acid molecule encoding a polypeptide comprising amino acids 1-91 of SEQ ID NO: 14, wherein said polypeptide binds Survivin;    (b) a nucleic acid molecule encoding a polypeptide comprising amino acids 85-125 of SEQ ID NO:14, wherein said polypeptide enhances cyclin B1/cdc2 kinase activity;    (c) a nucleic acid molecule encoding SEQ ID NO: 14; and    (d) a nucleic acid molecule that hybridizes to the complement of the nucleic acid molecule of (a), (b) or (c) under highly stringent hybridization conditions, and encodes a polypeptide that binds Survivin or enhances cyclin B1/cdc2 kinase activity.    
     
     
         2 . The isolated nucleic acid molecule of  claim 1 , wherein said polypeptide is encoded by a nucleotide sequence selected from the group consisting of: 
 (a) nucleotides 1-273 of SEQ ID NO:13;    (b) nucleotides 253-375 of SEQ ID NO:13; and    (c) SEQ ID NO: 13    
     
     
         3 . A vector comprising the nucleic acid molecule of  claim 1 .  
     
     
         4 . A recombinant cell comprising the nucleic acid molecule of  claim 1 .  
     
     
         5 . An oligonucleotide comprising at least 17 nucleotides capable of specifically hybridizing with a nucleotide sequence set forth in SEQ ID NO: 13 or its complement.  
     
     
         6 . The oligonucleotide of  claim 5 , wherein said oligonucleotide is labeled with a detectable label.  
     
     
         7 . A kit for detecting the presence of an SPB1 nucleic acid molecule, comprising at least one oligonucleotide according to  claim 5 .  
     
     
         8 - 11 . (canceled)  
     
     
         12 . A method for expression of an SBP1 polypeptide, said method comprising culturing the cell of  claim 4  under conditions suitable for expression of said SBP1.  
     
     
         13 - 34 . (canceled)