Full-length human cDNAs encoding potentially secreted proteins

Abstract

The invention concerns GENSET polynucleotides and polypeptides. Such GENSET products may be used as reagents in forensic analyses, as chromosome markers, as tissue/cell/organelle-specific markers, in the production of expression vectors. In addition, they may be used in screening and diagnosis assays for abnormal GENSET expression and/or biological activity and for screening compounds that may be used in the treatment of GENSET-related disorders.

1 . A composition of matter comprising: 
 a) an isolated polynucleotide, said polynucleotide comprising a nucleic acid sequence encoding: i) a polypeptide comprising an amino acid sequence having any one of the sequences shown as SEQ ID NOs: 242-482 or any one of the sequences of polypeptides encoded by the clone inserts of the deposited clone pool; or ii) a biologically active fragment of said polypeptide;    b) a nucleic acid sequence that has at least about 100 contiguous nucleotides of any one of the sequences shown as SEQ ID NOs: 1-241 or any one of the sequences of the clone inserts of the deposited clone pool;    c) a polynucleotide or nucleic acid sequence as set forth in a) or b) operably linked to a promoter;    d) an expression vector comprising a polynucleotide or nucleic acid sequence as set forth in a) or b) or c);    e) a recombinant host cell comprising a polynucleotide or nucleic acid sequence as set forth in a) or b) or c) or d);    f) a non-human transgenic animal comprising: i) a polynucleotide or nucleic acid sequence as set forth in a) or b) or c) or d) or ii) the host cell as set forth in e);    g) an isolated polypeptide or biologically active fragment thereof, said polypeptide comprising an amino acid sequence having any one of the sequences shown as SEQ ID NOs: 242-482 or any one of the sequences of polypeptides encoded by the clone inserts of the deposited clone pool; or    h) an isolated antibody that specifically binds to a polypeptide or biologically active fragment thereof, said polypeptide comprising an amino acid sequence having any one of the sequences shown as SEQ ID NOs: 242-482 or any one of the sequences of polypeptides encoded by the clone inserts of the deposited clone pool.    
     
     
         2 . The composition of matter according to  claim 1 , wherein said polynucleotide or nucleic acid sequence encodes a polypeptide that comprises a signal peptide.  
     
     
         3 . The composition of matter according to  claim 1 , wherein said polynucleotide encodes a polypeptide that is a mature protein.  
     
     
         4 . The composition of matter according to  claim 1 , wherein said polypeptide comprises a signal peptide.  
     
     
         5 . The composition of matter according to  claim 1 , wherein said polypeptide is a mature protein.  
     
     
         6 . A method of using a composition of matter according to  claim 1  for the production of a polypeptide; the binding of an antibody; determining if a polypeptide is present in a biological sample or expressed in a mammal; determining if a polypeptide is overexpressed or underexpressed in a mammal; or identifying candidate modulators of a polypeptide.  
     
     
         7 . The method according to  claim 6 , wherein said method of making a polypeptide comprises a) providing a population of host cells or a population of cells comprising: i) an isolated polynucleotide, said polynucleotide comprising a nucleic acid sequence encoding: A) a polypeptide comprising an amino acid sequence having any one of the sequences shown as SEQ ID NOs: 242-482 or any one of the sequences of polypeptides encoded by the clone inserts of the deposited clone pool; or B) a biologically active fragment of said polypeptide; ii) a nucleic acid sequence that has at least about 100 contiguous nucleotides of any one of the sequences shown as SEQ ID NOs: 1-241 or any one of the sequences of the clone inserts of the deposited clone pool; iii) a polynucleotide or nucleic acid sequence as set forth in i) or ii) operably linked to a promoter; or iv) an expression vector comprising a polynucleotide or nucleic acid as set forth in i) or ii) or iii); and b) culturing said population of host cells or said population of cells under conditions conducive to the production of said polypeptide within said population of host cells or population of cells.  
     
     
         8 . The method of  claim 7 , further comprising purifying said polypeptide from said population of host cells or said population of cells.  
     
     
         9 . The method according to  claim 6 , wherein said method of binding an antibody comprises the step of: contacting an antibody that specifically binds to a polypeptide or biologically active fragment thereof, said polypeptide comprising an amino acid sequence having any one of the sequences shown as SEQ ID NOs: 242-482 or any one of the sequences of polypeptides encoded by the clone inserts of the deposited clone pools with said polypeptide under conditions that allow binding of said antibody to said polypeptide.  
     
     
         10 . The method according to  claim 6 , wherein said method of determining whether a gene is expressed within a mammal comprises the steps of: a) providing a biological sample from said mammal b) contacting said biological sample with either of: i) a polynucleotide that hybridizes under stringent conditions a polynucleotide of  claim 1;  or ii) a polypeptide that specifically binds to a polypeptides as set forth in  claim 1;  and c) detecting the presence or absence of hybridization between said polynucleotide and an RNA species within said sample, or the presence or absence of binding of said polypeptide to a protein within said sample; wherein a detection of said hybridization or of said binding indicates that said gene is expressed within said mammal.  
     
     
         11 . The method of  claim 10 , wherein said polynucleotide is a primer, and wherein said hybridization is detected by detecting the presence of an amplification product comprising the sequence of said primer.  
     
     
         12 . The method of  claim 10 , wherein said polypeptide is an antibody.  
     
     
         13 . The method according to  claim 6 , wherein said method of determining whether a mammal has an elevated or reduced level of gene expression comprises the steps of: a) providing a biological sample from said mammal; and b) comparing the amount of a polypeptide or biologically active fragment thereof comprising an amino acid sequence having any one of the sequences shown as SEQ ID NOs: 242-482 or any one of the sequences of polypeptides encoded by the clone inserts of the deposited clone pool, or of an RNA species encoding said polypeptide, within said biological sample with a level detected in or expected from a control sample; wherein an increased amount of said polypeptide or said RNA species within said biological sample compared to said level detected in or expected from said control sample indicates that said mammal has an elevated level of said gene expression, and wherein a decreased amount of said polypeptide or said RNA species within said biological sample compared to said level detected in or expected from said control sample indicates that said mammal has a reduced level of said gene expression.  
     
     
         14 . A method of identifying a candidate modulator of a polypeptide, said method comprising: a) contacting a an isolated polypeptide or biologically active fragment thereof, said polypeptide comprising an amino acid sequence having any one of the sequences shown as SEQ ID NOs: 242-482 or any one of the sequences of polypeptides encoded by the clone inserts of the deposited clone pool with a test compound; and b) determining whether said compound specifically binds to said polypeptide; wherein a detection that said compound specifically binds to said polypeptide indicates that said compound is a candidate modulator of said polypeptide.