US2006117410A1PendingUtilityA1

Locked nucleic acid containing heteropolymers and related methods

53
Individually held — no corporate assignee on recordPriority: Jun 12, 2001Filed: Jan 25, 2006Published: Jun 1, 2006
Est. expiryJun 12, 2021(expired)· nominal 20-yr term from priority
Inventors:Stephen Goff
C07H 21/04
53
PatentIndex Score
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Cited by
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References
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Claims

Abstract

The present invention relates to methods of replacing nucleotides in target nucleic acid sequences using DNA-LNA chimeras. The method of the present invention provides for replacing a first nucleotide in a target sequence by exposing the target sequence to a DNA-LNA heteropolymer and thereby replacing the first nucleotide with a second nucleotide. The invention also features the DNA-LNA chimeras themselves as well as methods of making them.

Claims

exact text as granted — not AI-modified
1 . A method of replacing a first nucleotide in a target nucleic acid sequence comprising the step of exposing said target nucleic acid sequence to a DNA-LNA heteropolymer and thereby replacing said first nucleotide with a second nucleotide.  
     
     
         2 . The method of  claim 1 , wherein the first nucleotide is adenine.  
     
     
         3 . The method of  claim 1 , wherein the first nucleotide is thymine.  
     
     
         4 . The method of  claim 1 , wherein the first nucleotide is cytosine.  
     
     
         5 . The method of  claim 1 , wherein the first nucleotide is guanine.  
     
     
         6 . The method of  claim 1 , wherein the first nucleotide is uracil.  
     
     
         7 . The method of  claim 1 , wherein the first nucleotide is deoxyadenosine.  
     
     
         8 . The method of  claim 1 , wherein the first nucleotide is deoxycytidine.  
     
     
         9 . The method of  claim 1 , wherein the first nucleotide is deoxyguanosine.  
     
     
         10 . The method of  claim 1 , wherein the first nucleotide is uridine.  
     
     
         11 . The method of  claim 1 , wherein the first nucleotide is thymidine.  
     
     
         12 . The method of  claim 1 , wherein the second nucleotide is adenine.  
     
     
         13 . The method of  claim 1 , wherein the second nucleotide is thymine.  
     
     
         14 . The method of  claim 1 , wherein the second nucleotide is cytosine.  
     
     
         15 . The method of  claim 1 , wherein the second nucleotide is guanine.  
     
     
         16 . The method of  claim 1 , wherein the second nucleotide is uracil.  
     
     
         17 . The method of  claim 1 , wherein the second nucleotide is deoxyadenosine.  
     
     
         18 . The method of  claim 1 , wherein the second nucleotide is deoxycytidine.  
     
     
         19 . The method of  claim 1 , wherein the second nucleotide is deoxyguanosine.  
     
     
         20 . The method of  claim 1 , wherein the second nucleotide is uridine.  
     
     
         21 . The method of  claim 1 , wherein the second nucleotide is thymidine.  
     
     
         22 . The method of  claim 1 , wherein the target nucleic acid sequence is in or from an organism.  
     
     
         23 . The method of  claim 10 , wherein the organism is a plant.  
     
     
         24 . The method of  claim 11 , wherein the plant is maize.  
     
     
         25 . The method of  claim 11 , wherein the plant is a monocot.  
     
     
         26 . The method of  claim 11 , wherein the plant is a dicot.  
     
     
         27 . The method of  claim 11 , wherein the plant is tobacco.  
     
     
         28 . The method of  claim 10 , wherein the organism is an animal.  
     
     
         29 . The method of  claim 16 , wherein the animal is a mammal.  
     
     
         30 . The method of  claim 1 , wherein the target nucleic acid sequence is a DNA template that consist of deoxyribonucleotides.  
     
     
         31 . The method of  claim 1 , wherein the target nucleic acid sequence is an RNA template that consists of ribonucleotides.  
     
     
         32 . The method of  claim 1 , wherein the target nucleic acid sequence is a gene encoding AHAS.  
     
     
         33 . The method of  claim 1 , wherein the target nucleic acid sequence is an episomal target nucleic acid sequence.  
     
     
         34 . The method of  claim 1 , wherein the target nucleic acid sequence is a chromosomal target nucleic acid sequence.  
     
     
         35 . The method of  claim 1 , wherein the target nucleic acid sequence is an endogenous gene.  
     
     
         36 . The method of  claim 1 , wherein the target nucleic acid sequence is a transgene.  
     
     
         37 . The method of  claim 1 , wherein the target nucleic acid sequence is a recessive gene.  
     
     
         38 . The method of  claim 1 , wherein the target nucleic acid sequence is a dominant gene.  
     
     
         39 . The method of  claim 1 , wherein the step of exposing comprises using particle bombardment to deliver the DNA-LNA heteropolymer to said target nucleic acid sequence.  
     
     
         40 . The method of  claim 1 , wherein the step of exposing comprises using lipofection to deliver the DNA-LNA heteropolymer to said target nucleic acid sequence.  
     
     
         41 . The method of  claim 1 , wherein said method is used to improve a crop.  
     
     
         42 . The method of  claim 1 , wherein said method is used in a reverse genetics method.  
     
     
         43 . The method of  claim 1 , wherein the method confers a new property or trait on an organism.  
     
     
         44 . The method of  claim 31 , wherein the property or trait is herbicide resistance.  
     
     
         45 . The method of  claim 32 , wherein the herbicide is an imidazoline.  
     
     
         46 . The method of  claim 1 , wherein the method produces site-specific mutagenesis.  
     
     
         47 . The method of  claim 1 , wherein the method produces a gene knockout.  
     
     
         48 . The method of  claim 1 , wherein the method produces an allele replacement.  
     
     
         49 . The method of  claim 1 , wherein the method stably transmits a modified trait through mitosis and meiosis to progeny.  
     
     
         50 . The method of  claim 1 , wherein the method is performed in vivo.  
     
     
         51 . The method of  claim 1 , wherein the method is performed in vitro.  
     
     
         52 . The method of  claim 1 , further comprising the steps of synthesizing and purifying the DNA-LNA heteropolymer.  
     
     
         53 . The method of  claim 1 , further comprising growing cells to maturity.  
     
     
         54 . The method of  claim 1 , wherein said DNA-LNA heteropolymer is a DNA-LNA duplex nucleic acid.  
     
     
         55 . The method of  claim 1 , wherein said DNA-LNA heteropolymer is a DNA-LNA chimeric nucleic acid.  
     
     
         56 . The method of  claim 1 , wherein said DNA-LNA heteropolymer is a DNA-LNA heteroduplex nucleic acid.  
     
     
         57 . The method of  claim 1 , wherein said DNA-LNA heteropolymer is a DNA-LNA hairpin duplex nucleic acid.  
     
     
         58 . The method of  claim 1 , wherein said DNA-LNA heteropolymer is a DNA-LNA homo-duplex nucleic acid.  
     
     
         59 . A method of replacing a first nucleotide in a target nucleic acid sequence in one or more of a plurality of cells comprising the step of exposing said plurality of cells to one or more DNA-LNA heteropolymers and thereby replacing said first nucleotide with a second nucleotide in at least one or more of the plurality of cells.  
     
     
         60 - 111 . (canceled)

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