Human SDF-5 protein and compositions

Abstract

Purified human SDF-5 proteins and processes for producing them are disclosed. DNA molecules encoding the human SDF-5 proteins are also disclosed. The proteins may be used in regulating the binding of Wnt genes to their receptor. In preferred embodiments, the proteins may be used for inducing formation, growth, differentiation, proliferation and/or maintenance of chondrocytes and/or cartilage tissue, and for other tissue repair, such as pancreatic tissue repair.

1 . An isolated or recombinant polynucleotide comprising the nucleotide sequence from nucleotide #328 to #1140 of SEQ ID NO: 1.  
     
     
         2 . The polynucleotide of  claim 1 , wherein said polynucleotide comprises a nucleotide sequence selected from the group consisting of: 
 the nucleic sequence from nucleotide #256 to #1140 of SEQ ID NO: 1,    the nucleic sequence from nucleotide #307 to #1140 of SEQ ID NO: 1,    the nucleic sequence from nucleotide #310 to #1140 of SEQ ID NO: 1,    the nucleic sequence from nucleotide #313 to #1140 of SEQ ID NO: 1,    the nucleic sequence from nucleotide #316 to #1140 of SEQ ID NO: 1,    the nucleic sequence from nucleotide #319 to #1140 of SEQ ID NO: 1,    the nucleic sequence from nucleotide #322 to #1140 of SEQ ID NO: 1,    the nucleic sequence from nucleotide #325 to #1140 of SEQ ID NO: 1,    the nucleic sequence from nucleotide #256 to #1143 of SEQ ID NO: 1,    the nucleic sequence from nucleotide #307 to #1143 of SEQ ID NO: 1,    the nucleic sequence from nucleotide #310 to #1143 of SEQ ID NO: 1,    the nucleic sequence from nucleotide #313 to #1143 of SEQ ID NO: 1,    the nucleic sequence from nucleotide #316 to #1143 of SEQ ID NO: 1,    the nucleic sequence from nucleotide #319 to #1143 of SEQ ID NO: 1,    the nucleic sequence from nucleotide #322 to #1143 of SEQ ID NO: 1,    the nucleic sequence from nucleotide #325 to #1143 of SEQ ID NO: 1, and    the nucleic sequence from nucleotide #328 to #1143 of SEQ ID NO: 1.    
     
     
         3 . An isolated or recombinant polynucleotide comprising a nucleotide sequence encoding the amino acid sequence from amino acid #25 to #295 of SEQ ID NO: 2.  
     
     
         4 . The polynucleotide of  claim 3 , wherein said polynucleotide comprises a nucleotide sequence encoding an amino acid sequence selected from the group consisting of: 
 the amino acid sequence from amino acid #1 to #295 of SEQ ID NO:2,    the amino acid sequence from amino acid #18 to #295 of SEQ ID NO:2,    the amino acid sequence from amino acid #19 to #295 of SEQ ID NO:2,    the amino acid sequence from amino acid #20 to #295 of SEQ ID NO:2,    the amino acid sequence from amino acid #21 to #295 of SEQ ID NO:2,    the amino acid sequence from amino acid #22 to #295 of SEQ ID NO:2,    the amino acid sequence from amino acid #23 to #295 of SEQ ID NO:2, and    the amino acid sequence from amino acid #24 to #295 of SEQ ID NO:2.    
     
     
         5 . A vector comprising the polynucleotide of  claim 3 .  
     
     
         6 . A vector comprising the polynucleotide of  claim 3  operatively associated with an expression control sequence.  
     
     
         7 . A cell comprising the vector of  claim 6 .  
     
     
         8 . A cell comprising the polynucleotide of  claim 3 .  
     
     
         9 . An isolated or recombinant polynucleotide which is capable of hybridizing under stringent hybridization conditions to the polynucleotide of  claim 1  and encodes a protein having Frazzled activity.