Lipase powder, methods for producing the same and use thereof

Abstract

A lipase powder which is a granulated substance containing a lipase and a solid content of animal milk, a lipase composition wherein said lipase powder is immersed or impregnated in fatty oil, and a method for producing the lipase powder which comprises the step of adding animal milk or cream derived from the animal milk to an aqueous solution containing a lipase, and the step of spray-drying, freeze-drying or solvent-precipitating the mixture thereof are provided. According to the present invention, a lipase powder of which lipase activity and stability are improved can be provided.

1 . A lipase powder which is a granulated product containing a lipase and a solid content of animal milk.  
   
   
       2 . The lipase powder according to  claim 1 , wherein the lipase is a 1,3-specific lipase.  
   
   
       3 . The lipase powder according to  claim 2 , wherein the 1,3-specific lipase is a lipase derived from  Rhizomucor  sp. or  Alcaligenes  sp.  
   
   
       4 . The lipase powder according to  claim 2 , wherein the 1,3-specific lipase is a 1,3-specific lipase derived from  Rhizopus  sp. and  Thermomyces  sp.  
   
   
       5 . The lipase powder according to  claim 3 , wherein the 1,3-specific lipase is a 1,3-specific lipase derived from  Rhizomucor miehei.    
   
   
       6 . The lipase powder according to  claim 1 , wherein the solid content of animal milk is a solid content of cow milk or cream derived from cow milk.  
   
   
       7 . The lipase powder according to  claim 1  which has a water content of 10% by weight or less.  
   
   
       8 . The lipase powder according to  claim 1  which is obtained by adding animal milk or cream derived from animal milk to an aqueous solution containing a lipase, and spray-drying, freeze-drying or solvent-precipitating the mixture thereof.  
   
   
       9 . The lipase powder according to  claim 8  which is obtained by adjusting pH of the aqueous solution containing a lipase to 6 to 7.5 after adding the animal milk or the cream derived from animal milk thereto.  
   
   
       10 . The lipase powder according to  claim 1 , wherein 90% by weight or more of the lipase powder has a particle size of 1 to 100 μm.  
   
   
       11 . A lipase composition wherein the lipase powder according to  claim 1  is immersed or impregnated in fatty oil.  
   
   
       12 . The lipase composition according to  claim 11 , wherein the mass of the fatty oil in the lipase composition is 0.1 to 20 times mass of the lipase powder.  
   
   
       13 . A method for producing a lipase powder which comprises adding animal milk or cream derived from animal milk to an aqueous solution containing a lipase, and spray-drying, freeze-drying or solvent-precipitating the mixture thereof.  
   
   
       14 . The method according to  claim 13 , wherein the solid content of the added animal milk or cream derived from animal milk is 0.1 to 20 times mass of the solid content of the aqueous solution containing a lipase.  
   
   
       15 . The method according to  claim 13  which comprises adjusting pH of the aqueous solution containing a lipase to 6 to 7.5 after adding the animal milk or cream derived from animal milk thereto.  
   
   
       16 . The method according to  claim 13 , wherein the aqueous solution containing a lipase is a lipase culture solution from which a cell body is removed, or a purified culture solution thereof.  
   
   
       17 . The method according to  claim 13 , wherein the lipase is a lipase derived from  Rhizomucor  sp. or  Alcaligenes  sp.  
   
   
       18 . A lipase for trans-esterification or esterification containing the lipase powder according to  claim 1 .  
   
   
       19 . A trans-esterification method of fatty oil, which comprises using the lipase for the trans-esterification according to  claim 18.