Novel mixture and compounds from mycelia of antrodia camphorata and use thereof

Abstract

The present invention relates to novel mixtures and maleic and succinic acid derivatives from mycelium of Antrodia camphorata and the medical use thereof. The present invention relates to the composition or mycelium comprising the compounds of the invention.

1 . A mixture from mycelium of  Antrodia camphorata , which is prepared from water or organic solvent extract of mycelium of  Antrodia camphorata.    
     
     
         2 . The mixture of  claim 1 , wherein 
 the organic solvent is alcohol, ester, alkane or halogenated alkane.    
     
     
         3 . The mixture of  claim 2 , wherein the alcohol is ethanol.  
     
     
         4 . The mixture of  claim 1 , wherein the compound is 
 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-2,5-dione,    3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-1-ol-2,5-dione,    3R*,4S*-1-hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione, or    3R*,4R*-1-hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione.    
     
     
         5 . The mixture of  claim 1 , which decreases systolic blood pressure or increases high density lipoprotein.  
     
     
         6 . The mixture of  claim 1 , which has central cholinergic agonism, hepatoprotection, anti-inflammation or anti-tumor activity.  
     
     
         7 . The mixture of  claim 1 , wherein tumor is from the cells or tissues selected from the group consisting of liver, intestine, bone, blood, lymph and breast.  
     
     
         8 . A mycelium of  Antrodia camphorata  comprising the compounds of  claim 1 .  
     
     
         9 . The mycelium of  claim 1  wherein the compound is 
 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-2,5-dione,    3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-1-ol-2,5-dione,    3R*,4S*-1-hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione, or    3R*,4R*-1-hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione.    
     
     
         10 . A composition comprising: 
 an aqueous or organic solvent extract from mycelium of  Antrodia camphorata  and a suitable excipient.    
     
     
         11 . The composition according to  claim 10 , wherein: 
 the organic solvent is selected from a group consisting of alcohols, esters, alkanes, halogenated alkanes and mixtures thereof.    
     
     
         12 . The composition of  claim 11 , wherein:  
     
     
         12 . The composition of  claim 11 , wherein: 
 the organic solvent is ethanol.    
     
     
         13 . The composition according to  claim 10 , wherein:  
       the extract from mycelium of  Antrodia camphorata  includes a compound selected from a group consisting of 
 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-2,5-dione,  
 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-1-ol-2,5-dione,  
 3R*,4S*-1-hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione and  
 3R*,4R*-1-hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione.  
 
     
     
         14 . A method for the treating risk factors for cardiovascular disease comprising: 
 administering to a patient a therapeutically effective amount of a pharmaceutical composition, the composition including an extract from mycelium of  Antrodia camphorata  and a suitable excipient to effect a decrease in systolic blood pressure or an increase in high density lipoprotein of the patient.