Peptide sequence tags and method of using same

Abstract

Peptide sequence tags are identified and used to produce a class of global antibodies, which recognize all members of the GlnA protein sub-family with uniform specificity, regardless of the species of origin. The tags are used to create antibodies to the major GlnA protein of the Glutamine Synthetase enzyme catalyzing of carbon fixation. The antibodies have a range of applications as diagnostic detection reagents for the major environmental process of ammonia assimilation.

1 . A method for detecting the presence of a member of a GlnA protein (glutamine synthetase) in a sample comprising of: 
 (a) identifying and obtaining a peptide sequence tag conserved for GlnA protein, and exclusive to the GlnA protein, wherein said peptide sequence tag is    SEQ ID NO: 3    (b) utilizing the tag to elicit the production of antibodies; and    (c) using the antibodies to measure the concentration of a member of the GlnA protein in a sample.    
     
     
         2 . The peptide sequence tag  
       SEQ ID NO: 3.