Block-shaped scaffold for tissue engineering and production method thereof

Abstract

A block-shaped scaffold for a tissue engineering with improved shape stability and less volume change in water is produce by the steps of approximate-uniformly mixing the particle-shaped material having 100 to 2000 μm diameter with a solution, where a biodegradable polymer is dissolved with an organic solvent, freezing, drying it to remove the solvent, pulverizing thus obtained intermediate product, dissolving it with a liquid, where the biodegradable polymer is not dissolved, to remove the particle-shaped material taking thus obtained intermediate product into a mold, and pressing and heating it, the scaffold having ununiform and continuous holes occupying 20 to 80% in a cross-section area in a three-dimensional network structure having a small hole structure with 5 to 50 μm diameter, elastic coefficient being 0.1 to 2.5 MPa, and volume change being 95 to 105% when dipping it in water for 24 hours.

1 . A block-shaped scaffold for a tissue engineering comprising a bioabsorbable polymer material, wherein said scaffold has ununiform and continuous holes occuping 20 to 80% in a cross-section area in a three-dimensional network structure having a small hole structure with a hole diameter of 5 to 50 μm, an elastic coefficient being 0.1 to 2.5 MPa, and volume change being 95 to 105% when dipping it in water for 24 hours.  
   
   
       2 . The block-shaped scaffold for the tissue engineering as claimed in  claim 1 , 
 wherein the bioabsorbable polymer material is at least one kind selected from polyglycolic acid, polylactic acid, a copolymer of lactic acid and glycolic acid, poly-ε-caprolactone, a copolymer of lactic acid and ε-caprolactone, polyamino acid, polyortho ester, and a copolymer of those.    
   
   
       3 . A production method of the block-shaped scaffold for the tissue engineering, the method comprising, approximate-uniformly mixing a particle-shaped material having a particle diameter of 100 to 2000 μm with a solution in which a bioabsorbable polymer is dissolved with an organic solvent, where the particle-shaped material is not dissolved with said organic solvent but dissolved in a liquid in which the bioabsorbable polymer is not dissolved, freezing the mixture, drying it to remove said organic solvent, and producing thereby the polymer material containing a particle-shaped material and having the small hole structure where the hole diameter is 5 to 50 μm; pulverizing said produced polymer material, removing said particle-shaped material by dissolving it with a liquid in which the bioabsorbable polymer is not dissolved, passing it through a sieve, and producing thereby the bioabsorbable granular porous material having the particle diameter of 100 to 3000 μm; and taking said bioabsorbable granular porous material into a mold, pressing and heating it, producing thereby the block-shaped scaffold for the tissue engineering having ununiform and continuous holes occuping 20 to 80% in the cross-section area in the three-dimensional network structure having the small hole structure with the hole diameter of 5 to 50 μm, where the elastic coefficient is 0.1 to 2.5 MPa, and the volume change is 95 to 105% when dipping it in water for 24 hours.  
   
   
       4 . The production method of the block-shaped scaffold for the tissue engineering as claimed in  claim 3 , wherein at least one kind selected from polyglycolic acid, polylactic acid, a copolymer of lactic acid and glycolic acid, poly-ε-caprolactone, a copolymer of lactic acid and ε-caprolactone, polyamino acid, polyortho ester, and a copolymer of those, is used as the bioabsorbable polymer material.  
   
   
       5 . The production method of the block-shaped scaffold for the tissue engineering as claimed in  claim 3 , wherein at least one kind selected from chloroform, dichloromethane, carbon tetrachloride, acetone, dioxane, and tetrahydrofuran is used as the organic solvent.  
   
   
       6 . The production method of the block-shaped scaffold for the tissue engineering as claimed in  claim 4 , wherein at least one kind selected from chloroform, dichloromethane, carbon tetrachloride, acetone, dioxane, and tetrahydrofuran is used as the organic solvent.  
   
   
       7 . The production method of the block-shaped scaffold for the tissue engineering as claimed in  claim 3 , wherein the block-shaped scaffold for the tissue engineering is produced by heating the bioabsorbable granular porous material at 60 to 200° C. while keeping the volume in a state of being pressed at 500 to 3000 g/cm 2 .  
   
   
       8 . The production method of the block-shaped scaffold for the tissue engineering as claimed in  claim 4 , wherein the block-shaped scaffold for the tissue engineering is produced by heating the bioabsorbable granular porous material at 60 to 200° C. while keeping the volume in a state of being pressed at 500 to 3000 g/cm 2 .  
   
   
       9 . The production method of the block-shaped scaffold for the tissue engineering as claimed in  claim 5 , wherein the block-shaped scaffold for the tissue engineering is produced by heating the bioabsorbable granular porous material at 60 to 200° C. while keeping the volume in a state of being pressed at 500 to 3000 g/cm 2 .  
   
   
       10 . The production method of the block-shaped scaffold for the tissue engineering as claimed in  claim 6 , wherein the block-shaped scaffold for the tissue engineering is produced by heating the bioabsorbable granular porous material at 60 to 200° C. while keeping the volume in a state of being pressed at 500 to 3000 g/cm 2 .